Stabilisation of half MCM ring by Cdt1 during DNA insertion.

Szczegóły
Abstrakt

Tytuł:: Stabilisation of half MCM ring by Cdt1 during DNA insertion.
Autorzy:: Guerrero-Puigdevall M; Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IDIBGI), Parc Hospitalari Martí i Julià de Salt, Catalunya, Spain.
Fernandez-Fuentes N; Department of Biosciences, U Science Tech, Universitat de Vic-Universitat Central de Catalunya, Vic, Spain.
Frigola J; Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IDIBGI), Parc Hospitalari Martí i Julià de Salt, Catalunya, Spain. .
Źródło:: Nature communications [Nat Commun] 2021 Mar 19; Vol. 12 (1), pp. 1746. Date of Electronic Publication: 2021 Mar 19.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [London] : Nature Pub. Group
MeSH Terms:: Cell Cycle Proteins/*metabolism
DNA Replication/*physiology
DNA-Binding Proteins/*metabolism
Minichromosome Maintenance Proteins/*metabolism
Saccharomyces cerevisiae Proteins/*metabolism
Cell Cycle Proteins/chemistry ; Cell Cycle Proteins/genetics ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/genetics ; Minichromosome Maintenance Complex Component 6/chemistry ; Minichromosome Maintenance Complex Component 6/metabolism ; Minichromosome Maintenance Proteins/chemistry ; Minichromosome Maintenance Proteins/genetics ; Nuclear Proteins/metabolism ; Origin Recognition Complex/chemistry ; Origin Recognition Complex/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics
References:: Nature. 2013 Mar 21;495(7441):339-43. (PMID: 23474987)
Nat Struct Mol Biol. 2017 Mar;24(3):316-324. (PMID: 28191893)
J Biol Chem. 2010 Apr 23;285(17):12469-73. (PMID: 20202939)
Cell. 2015 Apr 23;161(3):513-525. (PMID: 25892223)
J Biol Chem. 2003 Feb 14;278(7):4491-9. (PMID: 12480933)
Genes Dev. 2014 Aug 1;28(15):1653-66. (PMID: 25085418)
Genes Dev. 2016 Jul 1;30(13):1492-502. (PMID: 27401552)
Nature. 2018 Jul;559(7713):217-222. (PMID: 29973722)
J Biol Chem. 2008 Mar 14;283(11):6817-25. (PMID: 18184650)
Nat Struct Mol Biol. 2013 Aug;20(8):944-51. (PMID: 23851460)
Mol Cell. 2013 May 23;50(4):577-88. (PMID: 23603117)
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20240-5. (PMID: 19910535)
Mol Cell. 2014 Sep 4;55(5):655-65. (PMID: 25087876)
Science. 2017 Jul 21;357(6348):314-318. (PMID: 28729513)
Mol Cell. 2014 Sep 4;55(5):666-77. (PMID: 25087873)
Nat Commun. 2017 Jun 23;8:15720. (PMID: 28643783)
Cell. 2009 Nov 13;139(4):719-30. (PMID: 19896182)
Crit Rev Biochem Mol Biol. 2017 Apr;52(2):107-144. (PMID: 28094588)
Nat Struct Mol Biol. 2014 Jan;21(1):20-5. (PMID: 24389553)
Nature. 2019 Nov;575(7784):704-710. (PMID: 31748745)
Genes (Basel). 2016 Dec 22;8(1):. (PMID: 28025526)
Cell Div. 2006 Oct 17;1:22. (PMID: 17042960)
Mol Cell. 2008 Jul 25;31(2):287-93. (PMID: 18657510)
Mol Cell. 2017 Jul 20;67(2):168-179. (PMID: 28732205)
Proc Natl Acad Sci U S A. 2020 Jul 28;117(30):17747-17756. (PMID: 32669428)
Nucleic Acids Res. 2012 Apr;40(7):3208-17. (PMID: 22140117)
J Cell Sci. 2012 Jan 1;125(Pt 1):209-19. (PMID: 22250202)
PLoS Genet. 2020 Aug 25;16(8):e1008988. (PMID: 32841231)
Nat Struct Mol Biol. 2017 Mar;24(3):300-308. (PMID: 28191894)
Substance Nomenclature:: 0 (CDC6 protein, S cerevisiae)
0 (Cell Cycle Proteins)
0 (DNA-Binding Proteins)
0 (Nuclear Proteins)
0 (ORC4 protein, S cerevisiae)
0 (ORC5 protein, S cerevisiae)
0 (Origin Recognition Complex)
0 (Saccharomyces cerevisiae Proteins)
0 (TAH11 protein, S cerevisiae)
EC 3.6.4.12 (MCM6 protein, S cerevisiae)
EC 3.6.4.12 (Minichromosome Maintenance Complex Component 6)
EC 3.6.4.12 (Minichromosome Maintenance Proteins)
Entry Date(s):: Date Created: 20210320 Date Completed: 20210405 Latest Revision: 20210417
Update Code:: 20240105
PubMed Central ID:: PMC7979726
DOI:: 10.1038/s41467-021-21932-8
PMID:: 33741931
: Czasopismo naukowe

Full Text Finder

Origin licensing ensures precise once per cell cycle replication in eukaryotic cells. The Origin Recognition Complex, Cdc6 and Cdt1 load Mcm2-7 helicase (MCM) into a double hexamer, bound around duplex DNA. The complex formed by ORC-Cdc6 bound to duplex DNA (OC) recruits the MCM-Cdt1 complex into the replication origins. Through the stacking of both complexes, the duplex DNA is inserted inside the helicase by an unknown mechanism. In this paper we show that the DNA insertion comes with a topological problem in the stacking of OC with MCM-Cdt1. Unless an essential, conserved C terminal winged helix domain (C-WHD) of Cdt1 is present, the MCM splits into two halves. The binding of this domain with the essential C-WHD of Mcm6, allows the latching between the MCM-Cdt1 and OC, through a conserved Orc5 AAA-lid interaction. Our work provides new insights into how DNA is inserted into the eukaryotic replicative helicase, through a series of synchronized events.

Informacja