Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Two Dimensional Anion Exchange-Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Disease Vaccine.

Tytuł :
Two Dimensional Anion Exchange-Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Disease Vaccine.
Autorzy :
Hossienizadeh SMJ; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
Bagheri M; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran; Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran.
Alizadeh M; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran; Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51664, Iran.
Rahimi M; Process Simulation and Control Research Laboratory, School of Pharmaceutical and Chemical Engineering, Shahid Beheshti University, Tehran, Iran.
Azimi SM; Foot and mouth Disease Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Kamalzade M; Department of Viral Vaccine Quality Control, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Es-Haghi A; Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran.
Ghassempour A; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran. Electronic address: .
Pokaż więcej
Źródło :
Journal of chromatography. A [J Chromatogr A] 2021 Apr 26; Vol. 1643, pp. 462070. Date of Electronic Publication: 2021 Mar 15.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Amsterdam ; New York : Elsevier, 1993-
MeSH Terms :
Models, Theoretical*
Chromatography, Gel/*methods
Chromatography, Ion Exchange/*methods
Viral Vaccines/*analysis
Animals ; Cell Line ; Cricetinae ; Dynamic Light Scattering ; Electrophoresis, Polyacrylamide Gel ; Foot-and-Mouth Disease/prevention & control ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Viral Vaccines/isolation & purification ; Virus Inactivation
Contributed Indexing :
Keywords: Chromatography modeling; Foot and mouth virus; MALDI-TOF MS; Non-structural proteins; Two dimensional chromatography
Substance Nomenclature :
0 (Viral Vaccines)
Entry Date(s) :
Date Created: 20210327 Date Completed: 20210504 Latest Revision: 20210504
Update Code :
20210506
DOI :
10.1016/j.chroma.2021.462070
PMID :
33773416
Czasopismo naukowe
The production of high-quality purified virus particles in high quantities for vaccine preparation requires a scalable purification procedure in the downstream step. A purification scheme based on combined strong anion-exchange and size exclusion chromatography (2D-AEC-SEC) was developed for the production of non-structural protein-free foot and mouth disease vaccine, and the whole procedure was accomplished with 77.9% virus yield. Additionally, a mathematical modeling and a simulation approach based on a plate model of chromatography were developed and matched with the experimental chromatography data to improve prediction of retention behavior and save time in the development of the downstream scale-up method. The purified pooled virus fraction obtained from the final polishing step had a purity higher than 85% based on analytical size exclusion analysis. Moreover, more than 90.1% of residual DNA (rDNA) was removed from the purified vaccine. The analysis of purified virus particles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS), high performance size exclusion chromatography (HP-SEC), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and transmission electron microscopy (TEM) provided clear evidence of purity and demonstrated that the final product is structurally spherical, intact particles qualified for formulation as a vaccine product.
(Copyright © 2021. Published by Elsevier B.V.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies