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Tytuł:
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Extraction and purification of cis/trans asarone from Acorus tatarinowii Schott: Accelerated solvent extraction and silver ion coordination high-speed counter-current chromatography.
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Autorzy:
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Zhu H; School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Ali I; School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan.
Hussain H; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany.
Hussain M; Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan.
Wang XB; Training Base of State Key Laboratory of Coal Science and Technology Jountly Constructed by Shanxi Province and Ministry of Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China.
Song X; School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Luo G; Shandong Wooking Instruments Co. Ltd., Dezhou 251500, China.
Zhang Z; Shandong Wooking Instruments Co. Ltd., Dezhou 251500, China; Jinan Hanon Instruments Co. Ltd., Jinan 250100, China.
Wang Z; Shandong Wooking Instruments Co. Ltd., Dezhou 251500, China; Jinan Hanon Instruments Co. Ltd., Jinan 250100, China.
Wang D; School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China. Electronic address: .
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Źródło:
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Journal of chromatography. A [J Chromatogr A] 2021 Apr 26; Vol. 1643, pp. 462080. Date of Electronic Publication: 2021 Mar 18.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam ; New York : Elsevier, 1993-
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MeSH Terms:
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Acorus/*chemistry
Anisoles/*analysis
Countercurrent Distribution/*methods
Acorus/metabolism ; Allylbenzene Derivatives ; Anisoles/isolation & purification ; Density Functional Theory ; Isomerism ; Liquid-Liquid Extraction ; Magnetic Resonance Spectroscopy ; Plant Extracts/chemistry ; Silver/chemistry ; Spectrometry, Mass, Electrospray Ionization
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Contributed Indexing:
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Keywords: Acorus tatarinowii Schott; DFT simulation; High-speed counter-current chromatography; Silver coordination; α/β-asarone
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Substance Nomenclature:
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0 (Allylbenzene Derivatives)
0 (Anisoles)
0 (Plant Extracts)
0 (asarone)
3M4G523W1G (Silver)
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Entry Date(s):
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Date Created: 20210402 Date Completed: 20210504 Latest Revision: 20211204
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Update Code:
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20240104
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DOI:
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10.1016/j.chroma.2021.462080
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PMID:
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33799073
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Acorus tatarinowii Schott is a traditional Chinese medicine used to treat memory and cognitive dysfunction. Because of their efficacy and lower toxic effects, research on α- and β-asarone, the phytoconstituents, has attracted attention owing to their remarkable pharmacological activities. Silver ion coordination complexation high-speed counter-current chromatography was used to separate these isomers from A. tatarinowii extract, coupled with accelerated solvent extraction. Accelerated solvent extraction parameters were investigated with single-factor and orthogonal testing. A two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:1:2:1, v/v) with 0.50 mol/L silver ions was selected for separation. From 2.0 g crude extract, 1.4 g of β-asarone and 0.09 g of α-asarone were obtained with purities over 98% by sequential sample loading in 20 h. The isolated compounds were identified by electrospray ionization mass spectrometry, 1 H and 13 C NMR. Silver ions significantly increased the separation factor and retention of the stationary phase. The chromatographic behavior indicated that cis-configuration was more strongly complexed with the silver ion. This was further demonstrated with the help of computational analysis. In conclusion, the established method could be employed to separate other cis-trans or E/Z isomers that form coordination complexes.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2021. Published by Elsevier B.V.)
