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Tytuł:
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The nephroprotective effects and mechanisms of rehmapicrogenin include ROS inhibition via an oestrogen-like pathway both in vivo and in vitro.
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Autorzy:
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Wang M; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Ke Y; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Li Y; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Shan Z; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Mi W; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Cao Y; Henan University of Chinese Medicine, Zhengzhou 450046, China.
Feng W; Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China. Electronic address: .
Zheng X; Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China. Electronic address: .
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Źródło:
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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2021 Jun; Vol. 138, pp. 111305. Date of Electronic Publication: 2021 Apr 02.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Paris : Editions Scientifiques Elsevier
Original Publication: New York, N.Y. : Masson Pub. USA, Inc., c1982-
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MeSH Terms:
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Estrogens*/metabolism
Acute Kidney Injury/*prevention & control
Drugs, Chinese Herbal/*therapeutic use
Estrogen Antagonists/*therapeutic use
Reactive Oxygen Species/*antagonists & inhibitors
Signal Transduction/*drug effects
Acute Kidney Injury/metabolism ; Acute Kidney Injury/pathology ; Animals ; Cell Line ; Cytoprotection/drug effects ; Cytoprotection/physiology ; Drugs, Chinese Herbal/pharmacology ; Estrogen Antagonists/pharmacology ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Rats ; Reactive Oxygen Species/metabolism ; Signal Transduction/physiology
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Contributed Indexing:
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Keywords: Adriamycin; CKD; Nrf2/ARE; Oestrogen receptor; Oxidative stress; Rehmapicrogenin
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Substance Nomenclature:
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0 (Drugs, Chinese Herbal)
0 (Estrogen Antagonists)
0 (Estrogens)
0 (Reactive Oxygen Species)
0 (rehmapicrogenin)
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Entry Date(s):
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Date Created: 20210406 Date Completed: 20210722 Latest Revision: 20210722
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Update Code:
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20240104
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DOI:
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10.1016/j.biopha.2021.111305
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PMID:
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33820633
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Background: The root of Rehmannia glutinosa (R. glutinosa) is commonly used in various traditional Chinese herbal formulae to ameliorate nephropathy; however, little is known about its active component(s) and mechanisms.
Aim: In the present study, we examined the protective effect and potential mechanism of rehmapicrogenin, a monomeric compound extracted from R. glutinosa, against Adriamycin (ADR)-induced nephropathy (AN) in vivo and in vitro.
Methods: In this study, an ADR-induced kidney injury model was employed to investigate the nephroprotective effects of rehmapicrogenin in mice. In vivo, ELISA kits, flow cytometry, haematoxylin-eosin staining, immunofluorescence techniques, and western blotting were used to evaluate the effect of rehmapicrogenin on kidney injury in mice. In vitro, the effects of rehmapicrogenin on NRK-52E cellular damage induced by ADR were determined using the 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The mechanism was investigated using ELISA kits, flow cytometry and In-Cell Western™ blotting.
Results: In vivo, rehmapicrogenin treatment significantly attenuated the pathological changes in the kidney induced by ADR; rescued weight, serum creatinine (Scr), blood urea nitrogen (BUN) and urine albumin (U-ALB) levels; reduced reactive oxygen species (ROS) accumulation; and decreased oxidative stress, the apoptosis rate, and cell survival in ADR-treated mice. Importantly, both in vivo and in vitro experimental results demonstrated that rehmapicrogenin regulates the Nrf2/ARE signalling pathway, the most important pathway for oxidative stress. Rehmapicrogenin attenuated ADR-induced kidney damage by reducing oxidative stress through the oestrogen receptor pathway. Moreover, after treatment with ICI 182780 (the oestrogen receptor-nonspecific antagonist Faslodex), the improvement induced by rehmapicrogenin was significantly reversed.
Conclusions: In conclusion, rehmapicrogenin attenuates kidney damage by reducing inflammatory factor release through the oestrogen signalling pathway.
(Copyright © 2021. Published by Elsevier Masson SAS.)
