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Tytuł:
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The role of canonical Wnt signaling in dentin bridge formation.
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Autorzy:
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Hara M; Department of Oral Diagnostics and Comprehensive Dentistry, Matsumoto Dental University Hospital, 1780 Hirooka-gobara, Shiojiri, Nagano, 399-0781, Japan.
Horibe K; Department of Oral Histology, Matsumoto Dental University, 1780 Hirooka-gobara, Shiojiri, Nagano, 399-0781, Japan.
Mori H; Department of Oral Diagnostics and Comprehensive Dentistry, Matsumoto Dental University Hospital, 1780 Hirooka-gobara, Shiojiri, Nagano, 399-0781, Japan.
Nakamura H; Department of Oral Histology, Matsumoto Dental University, 1780 Hirooka-gobara, Shiojiri, Nagano, 399-0781, Japan. Electronic address: .
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Źródło:
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Journal of oral biosciences [J Oral Biosci] 2021 Jun; Vol. 63 (2), pp. 199-209. Date of Electronic Publication: 2021 Apr 15.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: 2012- : Amsterdam : Elsevier B.V.
Original Publication: Tokyo : Japanese Association for Oral Biology, [2004]-
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MeSH Terms:
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Dentin*
Wnt Signaling Pathway*
Animals ; Cell Differentiation ; Dental Pulp Capping ; Mice ; Odontoblasts
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Contributed Indexing:
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Keywords: Dentin; Macrophages; Odontoblasts; Wnt signaling pathway
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Entry Date(s):
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Date Created: 20210412 Date Completed: 20210708 Latest Revision: 20210708
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Update Code:
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20240105
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DOI:
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10.1016/j.job.2021.03.003
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PMID:
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33845204
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Objective: Wnt signaling has been reported to be involved in dentin bridge formation. However, the detailed mechanism has not yet been clarified. We elucidated the localization of canonical Wnt signaling molecules during dentin bridge formation.
Methods: Pulp of the maxillary first molar in mice was exposed and directly capped with MTA cement. Maxillae were collected on the 1st, 4th, 7th, 14th, and 28th days after treatment. After μCT analysis, immunohistochemistry for Wnt3a, Wnt10a, β-catenin, F4/80, and osterix was performed in paraffin-embedded sections.
Results: On the 4th and 7th days after pulp capping, odontoblasts and dental pulp cells expressed Wnt3a, Wnt10a, and β-catenin. On the 14th day, reactionary dentin was formed around the pulp exposure area. Odontoblasts and dental pulp cells express Wnt3a, Wnt10a, and β-catenin. Additionally, F4/80- and Wnt10a-positive macrophages were observed at the center of the dental pulp. When the dentin bridge was formed on the 28th day, reparative odontoblasts expressed Wnt3a, β-catenin and osterix.
Conclusion: Wnt ligands derived from odontoblasts and dental pulp cells are important for the activation of odontoblasts and the differentiation of reparative odontoblasts during dentin bridge formation. Macrophage-derived Wnts are also involved in reparative odontoblast differentiation.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.
(Copyright © 2021 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)
