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Tytuł:
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Bimodular thrombin aptamers with two types of non-covalent locks.
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Autorzy:
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Alieva R; Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.
Novikov R; Engelhardt Institute of Molecular Biology RAS, Moscow, Russian Federation.
Tashlitsky V; Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.
Arutyunyan A; Belozersky Research Institute of Physical Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation.
Kopylov A; Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.
Zavyalova E; Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.
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Źródło:
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Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2021; Vol. 40 (5), pp. 559-577. Date of Electronic Publication: 2021 Apr 13.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Philadelphia : Taylor & Francis
Original Publication: Monticello, NY : Marcel Dekker, c2000-
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MeSH Terms:
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Aptamers, Nucleotide*
G-Quadruplexes
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Contributed Indexing:
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Keywords: Aptamer; DNA nanoconstruction; G-quadruplex; non-covalent lock; thrombin
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Substance Nomenclature:
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0 (Aptamers, Nucleotide)
145563-68-4 (thrombin aptamer)
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Entry Date(s):
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Date Created: 20210413 Date Completed: 20211103 Latest Revision: 20211103
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Update Code:
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20240104
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DOI:
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10.1080/15257770.2021.1910297
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PMID:
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33847237
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Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Here two types of self-assembling locks were used to create bimodular aptamer constructions. Well-known aptamer to thrombin was chosen as a model object. The assembly of duplex locks was more efficient at low concentrations. The functional activity of aptamer modules was nearly the same as in HD1. However, the affinity of bimodular aptamers with G-quadruplex locks to immobilized thrombin was 5-10 times higher.
