Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria.

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Tytuł:: Diluted Luria-Bertani medium vs. sewage sludge as growth media: comparison of community structure and diversity in the culturable bacteria.
Autorzy:: Yamamoto K; Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-Ku, Kitakyushu, Japan.
Toya S; Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-Ku, Kitakyushu, Japan.
Sabidi S; Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-Ku, Kitakyushu, Japan.
Hoshiko Y; Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-Ku, Kitakyushu, Japan.
Maeda T; Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-Ku, Kitakyushu, Japan. .
Źródło:: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 May; Vol. 105 (9), pp. 3787-3798. Date of Electronic Publication: 2021 Apr 15.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Berlin ; New York : Springer International, c1984-
MeSH Terms:: Bacteria*/genetics
Sewage*
Agar ; Biodiversity ; Culture Media
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Grant Information:: JPMJAS2004 Japan Science and Technology Agency
Contributed Indexing:: Keywords: Diluted media; Diverse colony formation; Environmental bacterial sources; Waste sewage sludge
Substance Nomenclature:: 0 (Culture Media)
0 (Sewage)
9002-18-0 (Agar)
Entry Date(s):: Date Created: 20210415 Date Completed: 20210514 Latest Revision: 20210514
Update Code:: 20240104
DOI:: 10.1007/s00253-021-11248-4
PMID:: 33856534
: Czasopismo naukowe

Pełny tekst

Because colony formation is essential to seek bacterial functions by the direct observation of phenotype, the diversification of colony formation for culturable bacteria is a big challenge in the research field of Environmental Biotechnology. In this study, the biodiversity of cultivable bacteria (colony or liquid culture) was compared by using Luria-Bertani (LB) medium and waste sewage sludge (WSS) under different dilutions and temperatures. When WSS was used as a bacterial source, whereas the highest number of colonies was found at the concentration of WSS (5%), a particular concentration of LB (10%) or WSS (1%) as a growth medium showed the best number of the operational taxonomic units (OTUs) of colonies. The results of bacterial community structure indicated that there are 1, 8, and 12 bacterial genera found uniquely in the agar plates of LB, 10% LB, and 5% WSS. By contrast, when palm oil mill effluent sludge was used as a bacterial source, the effect of dilution was different with WSS. When comparing the biodiversity between colonies and liquid culture, a high OTU value was observed in the colonies on the plate. In addition, 30°C showed the highest number of colonies in LB, 10% LB, and 5% WSS whereas the best OTUs were observed at 37°C for LB and 10% LB, and at 25°C for 5% WSS. This study demonstrates the diversification of cultivable bacteria through the number of OTUs in diluted LB medium and WSS, which is beneficial to isolate a unique bacterial strain.Key points• Impacts of diluted LB medium and WSS for colony formation were determined.• Difference of concentration of LB and WSS made different effects on colony formation.• Temperature change affected on diluted LB and WSS as media.

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