Clinical metagenomics assessments improve diagnosis and outcomes in community-acquired pneumonia.

Tytuł:: Clinical metagenomics assessments improve diagnosis and outcomes in community-acquired pneumonia.
Autorzy:: Xie F; Chinese People's Liberation Army General Hospital, Beijing, 100039, China.
Duan Z; Chinese People's Liberation Army General Hospital, Beijing, 100039, China.
Zeng W; Vision Medicals Center for Medical Research, Guangdong, China.; Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China.
Xie S; Vision Medicals Center for Medical Research, Guangdong, China.
Xie M; Vision Medicals Center for Medical Research, Guangdong, China.
Fu H; Chinese People's Liberation Army General Hospital, Beijing, 100039, China.
Ye Q; Vision Medicals Center for Medical Research, Guangdong, China.
Xu T; Vision Medicals Center for Medical Research, Guangdong, China. .; Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China. .
Xie L; Chinese People's Liberation Army General Hospital, Beijing, 100039, China. .
Źródło:: BMC infectious diseases [BMC Infect Dis] 2021 Apr 15; Vol. 21 (1), pp. 352. Date of Electronic Publication: 2021 Apr 15.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, [2001-
MeSH Terms:: Community-Acquired Infections/*diagnosis
Metagenomics/*methods
Pneumonia/*diagnosis
Adult ; Aged ; Aged, 80 and over ; Bronchoalveolar Lavage Fluid/microbiology ; Cohort Studies ; Community-Acquired Infections/microbiology ; DNA, Bacterial/chemistry ; DNA, Bacterial/metabolism ; Female ; High-Throughput Nucleotide Sequencing ; Humans ; Male ; Middle Aged ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Pneumonia/microbiology ; Sequence Analysis, DNA ; Sputum/microbiology ; Young Adult
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Grant Information:: 61976223 National Natural Science Foundation of China; 2018TM-03 Key Program of Translational Medicine of PLA General Hospital; 2019YFC0121703 Military Medical Innovation Research Project of PLA General Hospital
Contributed Indexing:: Keywords: Clinical metagenomics; Clinical study; Community-acquired pneumonia; Next-generation sequencing; Polymicrobial infections
Substance Nomenclature:: 0 (DNA, Bacterial)
Entry Date(s):: Date Created: 20210416 Date Completed: 20210430 Latest Revision: 20210430
Update Code:: 20240104
PubMed Central ID:: PMC8047593
DOI:: 10.1186/s12879-021-06039-1
PMID:: 33858378
: Czasopismo naukowe

Pełny tekst

Background: Identifying the causes of community-acquired pneumonia (CAP) is challenging due to the disease's complex etiology and the limitations of traditional microbiological diagnostic methods. Recent advances in next generation sequencing (NGS)-based metagenomics allow pan-pathogen detection in a single assay, and may have significant advantages over culture-based techniques.
Results: We conducted a cohort study of 159 CAP patients to assess the diagnostic performance of a clinical metagenomics assay and its impact on clinical management and patient outcomes. When compared to other techniques, clinical metagenomics detected more pathogens in more CAP cases, and identified a substantial number of polymicrobial infections. Moreover, metagenomics results led to changes in or confirmation of clinical management in 35 of 59 cases; these 35 cases also had significantly improved patient outcomes.
Conclusions: Clinical metagenomics could be a valuable tool for the diagnosis and treatment of CAP.
Trial Registration: Trial registration number with the Chinese Clinical Trial Registry: ChiCTR2100043628 .

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