Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as the sole carbon.
Sun H; College of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, China.
Wang L; Research Institute of Membrane Separation Technology of Shaanxi Province, Xi'an, China.
Nie H; College of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, China.
Diwu Z; Shaanxi Key Laboratory of Membrane Separation, Xi'an, China.
Nie M; Research Institute of Membrane Separation Technology of Shaanxi Province, Xi'an, China.
Zhang B; College of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, China.
Biotechnology progress [Biotechnol Prog] 2021 Apr 19, pp. e3155. Date of Electronic Publication: 2021 Apr 19.
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Publication: <2010-> : Hoboken, NJ : Wiley-Blackwell
Original Publication: [New York, N.Y. : American Institute of Chemical Engineers, c1985-
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Grant Information :
18JK0449 Special Scientific Research Foundation of Education Department of Shaanxi Provincial Government of China; 2017ZDCXL-GY-07-03 Shaanxi Province Key Industrial Cluster Project; 2017KCT-19-01 Innovation Team Plan of Shaanxi Province Key Science and Technology
Contributed Indexing :
Keywords: Plackett-Burman design; Pseudomonas aeruginosa NY3; Rhamnolipid; congeners; response surface methodology
Entry Date(s) :
Date Created: 20210419 Latest Revision: 20210429
Update Code :
Yield and cost are two major factors limiting the widespread use of rhamnolipids (RLs). In the present study, waste frying oil (WFO) was used as the sole carbon source to produce environmentally friendly RLs by Pseudomonas aeruginosa NY3. The Plackett-Burman design (PBD) and Box-Behnken design (BBD) methods were used to maximize the production yield of RL. The PBD results showed that the concentrations of NaNO 3 , Na 2 HPO 4 , and trace elements were the key factors affecting the yield of RL. Furthermore, the BBD results showed that at NaNO 3 , Na 2 HPO 4 , and trace elements concentrations were 4.95, 0.66, and 0.64 mL/L, respectively, the average RL yield reached 9.15 ± 0.52 g/L, 1.58-fold higher than that observed before optimization. Fourier transform infrared spectroscopy (FTIR) and liquid chromatography-ion trap-time of flight mass spectrometry (LCMS-IT-TOF) were used to elucidate the diversity of RL congeners. The results showed that, after optimization, the RL congener diversity increased, and the major RL constituent was converted from di-RLs (64.04%) to mono-RLs (60.44%). These results suggested that the concentrations of the components contained in the culture medium of P. aeruginosa NY3 influenced not only the yield of RL, but also its congener distribution.
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