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Tytuł:
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Lipopolysaccharide from biofilm-forming Pseudomonas aeruginosa PAO1 induces macrophage hyperinflammatory responses.
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Autorzy:
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Wang S; Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Xiang D; Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Tian F; Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Ni M; Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
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Źródło:
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Journal of medical microbiology [J Med Microbiol] 2021 Apr; Vol. 70 (4).
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2015- : London : Microbiology Society
Original Publication: 1968-1995: Edinburgh, Livingstone.
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MeSH Terms:
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Biofilms/*growth & development
Lipopolysaccharides/*pharmacology
Macrophages/*drug effects
Pseudomonas aeruginosa/*chemistry
Animals ; Blotting, Western ; Cytokines/analysis ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique ; Humans ; Macrophages/pathology ; Mice ; Microscopy, Fluorescence ; Pseudomonas aeruginosa/physiology ; RAW 264.7 Cells ; RNA, Bacterial/genetics ; RNA, Bacterial/isolation & purification ; THP-1 Cells
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References:
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Contributed Indexing:
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Keywords: Pseudomonas aeruginosa; biofilm; lipopolysaccharide; macrophages; polarization
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Substance Nomenclature:
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0 (Cytokines)
0 (Lipopolysaccharides)
0 (RNA, Bacterial)
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Entry Date(s):
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Date Created: 20210428 Date Completed: 20210504 Latest Revision: 20210722
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Update Code:
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20240104
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PubMed Central ID:
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PMC8289208
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DOI:
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10.1099/jmm.0.001352
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PMID:
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33909550
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Introduction. Macrophages polarization is essential in infection control. Llipopolysaccharide (LPS) plays an essential role in host innate immune system-pathogen interaction. The LPS structure of Pseudomonas aeruginosa modifies in the adaptation of this pathogen to biofilm-related chronic infection. Gap statement. There have been several studies on LPS induced polarization of human and mouse macrophages with different results. And it was reported that the lipid A structure of the LPS derived from biofilm-forming Pseudomonas aeruginosa strain PAO1 was modified. Aim. This study aimed to investigate the effect and the involved pathway of LPS from biofilm-forming PAO1 on human and murine macrophage polarization. Methodology. LPS was isolated from biofilm-forming and planktonic PAO1 and quantified. Then the LPS was added to PMA-differentiated human macrophage THP-1 cells and Raw264.7 murine macrophage cells. The expression of iNOS, Arg-1, IL4 , TNF-α, CCL3 , and CCL22 was analysed in the different cell lines. The expression of TICAM-1 and MyD88 in human THP-1 macrophages was quantified by Western blot. PAO1 infected macrophages at different polarization states, and the intracellular bacterial growth in macrophages was evaluated. Results. LPS from biofilm-forming PAO1 induced more marked hyperinflammatory responses in THP-1 and Raw264.7 macrophages than LPS derived from planktonic PAO1, and these responses were related to the up-regulation of MyD88. Intracellular growth of PAO1 was significantly increased in THP-1 macrophages polarized by LPS from biofilm-forming PAO1, but decreased both in THP-1 and Raw264.7 macrophages polarized by LPS from planktonic PAO1. Conclusion. The presented in vitro study indicates that LPS derived from biofilm-forming PAO1 induces enhanced M1 polarization in human and murine macrophage cell lines than LPS from planktonic PAO1.