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Tytuł pozycji:

Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1.

Tytuł:
Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1.
Autorzy:
Ellefsen CF; Department of Pharmacy, University of Oslo, P.O.Box 1068 Blindern, NO-0316, Oslo, Norway. Electronic address: .
Wold CW; Department of Pharmacy, University of Oslo, P.O.Box 1068 Blindern, NO-0316, Oslo, Norway.
Wilkins AL; School of Science and Engineering, University of Waikato, Private Bag 3105, Hamilton, 3240, New Zealand.
Rise F; Department of Chemistry, University of Oslo, P.O.Box 1033 Blindern, NO-0315, Oslo, Norway.
Samuelsen ABC; Department of Pharmacy, University of Oslo, P.O.Box 1068 Blindern, NO-0316, Oslo, Norway.
Źródło:
Carbohydrate polymers [Carbohydr Polym] 2021 Jul 15; Vol. 264, pp. 117991. Date of Electronic Publication: 2021 Mar 26.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: <1992-> : Barking : Elsevier Applied Science Publishers
Original Publication: London [Eng.] : Applied Science Publishers, c1981-
MeSH Terms:
Fruiting Bodies, Fungal/*chemistry
Fungal Polysaccharides/*metabolism
Lectins, C-Type/*metabolism
Pleurotus/*chemistry
Toll-Like Receptor 2/*metabolism
Animals ; Cell Line ; Cytokines/metabolism ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Fungal Polysaccharides/immunology ; Humans ; Immunomodulation ; Magnetic Resonance Spectroscopy/methods ; Mice ; Nitric Oxide/metabolism ; Polysaccharides/immunology ; Polysaccharides/metabolism ; Toll-Like Receptors/metabolism ; Water/chemistry ; beta-Glucans/immunology ; beta-Glucans/metabolism
Contributed Indexing:
Keywords: Dectin-1; Mannogalactan; Pleurotus eryngii; TLR-2; β-Glucan
Substance Nomenclature:
0 (Cytokines)
0 (Fungal Polysaccharides)
0 (Lectins, C-Type)
0 (Polysaccharides)
0 (Toll-Like Receptor 2)
0 (Toll-Like Receptors)
0 (beta-Glucans)
0 (dectin 1)
0 (mannoglucan)
059QF0KO0R (Water)
31C4KY9ESH (Nitric Oxide)
SCR Organism:
Pleurotus eryngii
Entry Date(s):
Date Created: 20210429 Date Completed: 20210921 Latest Revision: 20210921
Update Code:
20240104
DOI:
10.1016/j.carbpol.2021.117991
PMID:
33910729
Czasopismo naukowe
The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like receptors (TLR) and dectin-1. In the present study, Pleurotus eryngii polysaccharide fractions containing a 3-O methylated mannogalactan and (1→3)/(1→6)-β-d-glucans were isolated and extensively characterized by 2D NMR and methylation analysis. Traces of a (1→3)-α-d-glucan and a (1→2)-α-d-mannan were also observed. Affinity for TLR2, TLR2-TLR6 and dectin-1 using HEK-cells expressing the relevant receptor genes was tested. PeWN, containing the 3-O methylated mannogalactan, was inactive towards TLR2, whereas fraction PeWB, containing more β-glucan, activated the TLR2-TLR6 heterodimer. Activation of the human β-glucan receptor dectin-1 correlated with the amount of β-glucan in each fraction. Nitric oxide and cytokine supernatant levels of D2SC/1 dendritic cells stimulated with the P. eryngii fractions and interferon-γ were low to moderate. The results indicate that the immunomodulatory activity of water-soluble P. eryngii polysaccharide fractions is modest.
(Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

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