Rapid Sorting of Fucoxanthin-Producing Phaeodactylum tricornutum Mutants by Flow Cytometry.

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Tytuł:: Rapid Sorting of Fucoxanthin-Producing Phaeodactylum tricornutum Mutants by Flow Cytometry.
Autorzy:: Fan Y; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.; University of Chinese Academy of Sciences, Beijing 100049, China.; Shandong Energy Institute, Qingdao 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
Ding XT; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.; Shandong Energy Institute, Qingdao 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
Wang LJ; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Jiang EY; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.; Shandong Energy Institute, Qingdao 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
Van PN; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.; Shandong Energy Institute, Qingdao 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
Li FL; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.; Shandong Energy Institute, Qingdao 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
Źródło:: Marine drugs [Mar Drugs] 2021 Apr 17; Vol. 19 (4). Date of Electronic Publication: 2021 Apr 17.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2003]-
MeSH Terms:: Flow Cytometry*
Mutation*
Diatoms/*metabolism
Xanthophylls/*metabolism
Biomarkers/metabolism ; Diatoms/genetics ; Diatoms/radiation effects ; Heavy Ions ; High-Throughput Screening Assays ; Time Factors ; Workflow
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Grant Information:: 2019YFD0901904；2018YFE0107200 National Key Research and Development Program of China; 2019GHY112001 Key Technology Research and Development Program of Shandong; 31973007 National Natural Science Foundation of China
Contributed Indexing:: Keywords: Phaeodactylum tricornutum; flow cytometry; fucoxanthin; heavy ion irradiation
Substance Nomenclature:: 0 (Biomarkers)
0 (Xanthophylls)
06O0TC0VSM (fucoxanthin)
Entry Date(s):: Date Created: 20210430 Date Completed: 20210607 Latest Revision: 20210607
Update Code:: 20240104
PubMed Central ID:: PMC8072577
DOI:: 10.3390/md19040228
PMID:: 33920502
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Fucoxanthin, which is widely found in seaweeds and diatoms, has many benefits to human health, such as anti-diabetes, anti-obesity, and anti-inflammatory physiological activities. However, the low content of fucoxanthin in brown algae and diatoms limits the commercialization of this product. In this study, we introduced an excitation light at 488 nm to analyze the emitted fluorescence of Phaeodactylum tricornutum , a diatom model organism rich in fucoxanthin. We observed a unique spectrum peak at 710 nm and found a linear correlation between fucoxanthin content and the mean fluorescence intensity. We subsequently used flow cytometry to screen high-fucoxanthin-content mutants created by heavy ion irradiation. After 20 days of cultivation, the fucoxanthin content of sorted cells was 25.5% higher than in the wild type. This method provides an efficient, rapid, and high-throughput approach to screen fucoxanthin-overproducing mutants.

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