-
Tytuł:
-
Noncanonical DNA polymerization by aminoadenine-based siphoviruses.
-
Autorzy:
-
Pezo V; Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ. Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, France.
Jaziri F; Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ. Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, France.
Bourguignon PY; Werkstatt fuer Potenzielle Genetik, Naunynstrasse 30, 10997 Berlin, Germany.; TESSSI, 81 Rue Réaumur, 75002 Paris, France.
Louis D; Alderys, 86 Rue de Paris, 91400 Orsay, France.
Jacobs-Sera D; Department of Biological Sciences, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA 15260 USA.
Rozenski J; Laboratory of Medicinal Chemistry, Rega Institute for Biomedical Research, KU Leuven, Herestraat 49, Box 1041, 3000 Leuven, Belgium.
Pochet S; Organic Chemistry, CNRS UMR3523, Department of Chemistry and Biocatalysis, Institut Pasteur, 25-28 Rue du Docteur Roux, 75015 Paris, France.
Herdewijn P; Laboratory of Medicinal Chemistry, Rega Institute for Biomedical Research, KU Leuven, Herestraat 49, Box 1041, 3000 Leuven, Belgium.
Hatfull GF; Department of Biological Sciences, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA 15260 USA.
Kaminski PA; Biology of Gram-Positive Pathogens, CNRS URL3526, Department of Microbiology, Institut Pasteur, 25-28 Rue du Docteur Roux, 75015 Paris, France.
Marliere P; Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ. Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, 91057 Evry, France. .; TESSSI, 81 Rue Réaumur, 75002 Paris, France.
-
Źródło:
-
Science (New York, N.Y.) [Science] 2021 Apr 30; Vol. 372 (6541), pp. 520-524.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
-
MeSH Terms:
-
DNA Replication*
Polymerization*
2-Aminopurine/*analogs & derivatives
DNA, Viral/*biosynthesis
DNA-Directed DNA Polymerase/*chemistry
Siphoviridae/*chemistry
Siphoviridae/*enzymology
Viral Nonstructural Proteins/*chemistry
2-Aminopurine/chemistry ; DNA-Directed DNA Polymerase/classification ; DNA-Directed DNA Polymerase/genetics ; Genome, Viral ; Phylogeny ; Siphoviridae/genetics ; Viral Nonstructural Proteins/classification ; Viral Nonstructural Proteins/genetics
-
Substance Nomenclature:
-
0 (DNA, Viral)
0 (Viral Nonstructural Proteins)
452-06-2 (2-Aminopurine)
49P95BAU4Z (2,6-diaminopurine)
EC 2.7.7.7 (DNA-Directed DNA Polymerase)
-
Entry Date(s):
-
Date Created: 20210430 Date Completed: 20210513 Latest Revision: 20210521
-
Update Code:
-
20240104
-
DOI:
-
10.1126/science.abe6542
-
PMID:
-
33926956
-
Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3'-exonuclease but lacks 5'-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution.
(Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.
