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Tytuł:
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Single-chain antibody-decorated Au .
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Autorzy:
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Farahavar G; Pharmaceutical Nanotechnology Department, Shiraz University of Medical Sciences, Shiraz 71345, Iran. Electronic address: gh_.
Abolmaali SS; Pharmaceutical Nanotechnology Department, Shiraz University of Medical Sciences, Shiraz 71345, Iran; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 71345, Iran. Electronic address: .
Nejatollahi F; Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: .
Safaie A; Faculty of Science, Department of Physics, Shiraz University, Shiraz 71454, Iran. Electronic address: .
Javanmardi S; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 71345, Iran. Electronic address: .
Khajeh Zadeh H; Department of Photonic-Laser, Shiraz University of Technology, Shiraz, Iran. Electronic address: .
Yousefi R; Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran. Electronic address: .
Nadgaran H; Faculty of Science, Department of Physics, Shiraz University, Shiraz 71454, Iran. Electronic address: .
Mohammadi-Samani S; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 71345, Iran; Department of Pharmaceutics, Shiraz University of Medical Sciences, Shiraz 71345, Iran. Electronic address: .
Tamaddon AM; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz 71345, Iran. Electronic address: .
Ahadian S; Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90024, USA; Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: .
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Źródło:
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Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2021 May; Vol. 124, pp. 112086. Date of Electronic Publication: 2021 Mar 31.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier
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MeSH Terms:
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Hyperthermia, Induced*
Melanoma*/diagnostic imaging
Melanoma*/therapy
Gold ; Humans ; Photothermal Therapy ; Spectrum Analysis, Raman ; Theranostic Nanomedicine
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Contributed Indexing:
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Keywords: Immuno-liposomal layer coated AuNCs; Melanoma; Photothermal therapy; SERS imaging; Theranostic
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Substance Nomenclature:
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7440-57-5 (Gold)
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Entry Date(s):
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Date Created: 20210505 Date Completed: 20210514 Latest Revision: 20210514
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Update Code:
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20240104
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DOI:
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10.1016/j.msec.2021.112086
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PMID:
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33947576
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The development of theranostic platforms combining surface-enhanced Raman spectroscopy (SERS) imaging with NIR-stimulated photothermal therapy (PTT) is of utmost importance for the precise diagnosis and selective treatment of cancers, especially in superficial solid tumors. For this purpose, a versatile theranostic nanoprobe of liposomal layer-coated Au nanocages (AuNCs) was decorated with an anti-MUC18 single-chain antibody (scFv). 4-mercapto benzoic acid (p-MBA)-labeled AuNCs (p-AuNCs) were coated by a liposomal layer (p-AuNCs@lip), followed by conjugating anti-MUC18 scFv via post-insertion method to form immuno-liposomal layer-coated AuNCs (p-AuNCs@scFv-lip). Physicochemical characterizations of the p-AuNCs@scFv-lip were investigated by transmission electron microscopy (TEM) and UV-vis and Raman spectroscopy. Furthermore, the targeting ability and theranostic efficiency of the nanoprobe were evaluated for specific diagnosis and treatment of cancerous melanoma cells by flow cytometry, SERS mapping, and live/dead assay. The formation of lipid layer on p-AuNCs surface was confirmed by TEM imaging. After decorating the liposomal layer with scFv, a relevant red shift was observed in the UV-vis spectrum. Moreover, p-AuNCs@lip presented characteristic peaks in the Raman spectrum, which exhibited only a minor change after scFv conjugation (p-AuNCs@scFv-lip). Interestingly, the cellular uptake of AuNCs@scFv-lip by A375 cell line (MUC18 + ) showed a 24-fold enhancement compared with SKBR3 cells (MUC18 - ). AuNCs@scFv-lip specifically identified A375 cells from SKBR cells via SERS mapping and effectively killed A375 cells through the PTT mechanism. Taken together, this theranostic platform can provide a promising tool for both in situ diagnosis and remote-controlled thermal ablation of cancer cells.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
