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Tytuł:
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Evaluation of Cellular and Serological Responses to Acute SARS-CoV-2 Infection Demonstrates the Functional Importance of the Receptor-Binding Domain.
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Autorzy:
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Mantus G; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA.; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.
Nyhoff LE; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA.; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.
Kauffman RC; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA.; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.
Edara VV; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.; Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA.; Yerkes National Primate Research Center, Atlanta, GA.
Lai L; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.; Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA.; Yerkes National Primate Research Center, Atlanta, GA.
Floyd K; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.; Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA.; Yerkes National Primate Research Center, Atlanta, GA.
Shi PY; Department of Microbiology and Immunology, Institute for Human Infection and Immunity, World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX.
Menachery VD; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX.
Edupuganti S; Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Decatur, Atlanta, GA; and.
Scherer EM; Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Decatur, Atlanta, GA; and.
Kay A; Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Decatur, Atlanta, GA; and.
McNair N; Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Decatur, Atlanta, GA; and.
Anderson EJ; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA.
Rouphael N; Hope Clinic of the Emory Vaccine Center, Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine Decatur, Atlanta, GA; and.
Ahmed R; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
Suthar MS; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA.; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.; Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA.
Wrammert J; Centers for Childhood Infections and Vaccines, Children's Healthcare of Atlanta and Emory University, Department of Pediatrics, Atlanta, GA; .; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA.
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Źródło:
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Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2021 Jun 01; Vol. 206 (11), pp. 2605-2613. Date of Electronic Publication: 2021 May 05.
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Typ publikacji:
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Evaluation Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Bethesda, MD : American Association of Immunologists
Original Publication: Baltimore : Williams & Wilkins, c1950-
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MeSH Terms:
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Immunity, Cellular*
Immunologic Memory*
B-Lymphocytes/*immunology
COVID-19/*immunology
Nucleocapsid Proteins/*immunology
SARS-CoV-2/*immunology
Spike Glycoprotein, Coronavirus/*immunology
Acute Disease ; Cell Line ; Female ; Humans ; Male ; Protein Domains
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References:
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Grant Information:
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U01 AI150747 United States AI NIAID NIH HHS; P51 OD011132 United States OD NIH HHS; R00 AG049092 United States AG NIA NIH HHS; U19 AI057266 United States AI NIAID NIH HHS; UM1 AI148684 United States AI NIAID NIH HHS; R24 AI120942 United States AI NIAID NIH HHS
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Substance Nomenclature:
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0 (Nucleocapsid Proteins)
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
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Entry Date(s):
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Date Created: 20210506 Date Completed: 20210607 Latest Revision: 20220716
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Update Code:
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20240104
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PubMed Central ID:
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PMC8482837
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DOI:
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10.4049/jimmunol.2001420
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PMID:
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33952616
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The factors that control the development of an effective immune response to the recently emerged SARS-CoV-2 virus are poorly understood. In this study, we provide a cross-sectional analysis of the dynamics of B cell responses to SARS-CoV-2 infection in hospitalized COVID-19 patients. We observe changes in B cell subsets consistent with a robust humoral immune response, including significant expansion of plasmablasts and activated receptor-binding domain (RBD)-specific memory B cell populations. We observe elevated titers of Abs to SARS-CoV-2 RBD, full-length Spike, and nucleoprotein over the course of infection, with higher levels of RBD-specific IgG correlating with increased serum neutralization. Depletion of RBD-specific Abs from serum removed a major portion of neutralizing activity in most individuals. Some donors did retain significant residual neutralization activity, suggesting a potential Ab subset targeting non-RBD epitopes. Taken together, these findings are instructive for future vaccine design and mAb strategies.
(Copyright © 2021 by The American Association of Immunologists, Inc.)
