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Tytuł pozycji:

Spatial control of gene expression in flies using bacterially derived binary transactivation systems.

Tytuł:
Spatial control of gene expression in flies using bacterially derived binary transactivation systems.
Autorzy:
Gamez S; Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, California, USA.
Vesga LC; Group for Research in Biochemistry and Microbiology (Grupo de Investigación en Bioquímica Y Microbiología-GIBIM), School of Chemistry, Universidad Industrial de Santander, Bucaramanga, Colombia.
Mendez-Sanchez SC; Group for Research in Biochemistry and Microbiology (Grupo de Investigación en Bioquímica Y Microbiología-GIBIM), School of Chemistry, Universidad Industrial de Santander, Bucaramanga, Colombia.
Akbari OS; Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, California, USA.
Źródło:
Insect molecular biology [Insect Mol Biol] 2021 Oct; Vol. 30 (5), pp. 461-471. Date of Electronic Publication: 2021 May 24.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
Język:
English
Imprint Name(s):
Publication: Oxford : Blackwell Scientific For The Royal Entomological Society
Original Publication: Oxford : Published for the Royal Entomological Society by Blackwell Scientific Publications, c1992-
MeSH Terms:
Bacterial Proteins*/genetics
Drosophila melanogaster*/genetics
Gene Expression Regulation*
Operon*
Transcriptional Activation*
Animals
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Grant Information:
DP2 AI152071 United States AI NIAID NIH HHS; R01 AI151004 United States AI NIAID NIH HHS
Contributed Indexing:
Keywords: Caulobacter crescentus; Drosophila melanogaster; Pseudomonas putida; Streptomyces coelicolor; binary expression system; cymTA; pipTA; tTA; transactivators; ttgTA; vanTA
Substance Nomenclature:
0 (Bacterial Proteins)
Entry Date(s):
Date Created: 20210508 Date Completed: 20211124 Latest Revision: 20221003
Update Code:
20240105
PubMed Central ID:
PMC8459377
DOI:
10.1111/imb.12717
PMID:
33963794
Czasopismo naukowe
Controlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo; however, as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. To expand this molecular genetic toolbox, we tested multiple bacterially derived binary transactivational systems in Drosophila melanogaster including the p-CymR operon from Pseudomonas putida, PipR operon from Streptomyces coelicolor, TtgR operon from Pseudomonas putida and the VanR operon from Caulobacter crescentus. Our work provides the first characterization of these systems in an animal model in vivo. For each system, we demonstrate robust tissue-specific spatial transactivation of reporter gene expression, enabling future studies to exploit these transactivational systems for molecular genetic studies.
(© 2021 Royal Entomological Society.)

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