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Tytuł pozycji:

Transcription factor FOXC1 positively regulates SFRP1 expression in androgenetic alopecia.

Tytuł:
Transcription factor FOXC1 positively regulates SFRP1 expression in androgenetic alopecia.
Autorzy:
Zhou LB; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Cao Q; Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Ding Q; Department of Dermatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Sun WL; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Li ZY; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Zhao M; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Lin XW; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Zhou GP; Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.
Fan WX; Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China. Electronic address: .
Źródło:
Experimental cell research [Exp Cell Res] 2021 Jul 01; Vol. 404 (1), pp. 112618. Date of Electronic Publication: 2021 May 07.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: Orlando Fl : Academic Press
Original Publication: New York, Academic Press.
MeSH Terms:
Alopecia/*metabolism
Forkhead Transcription Factors/*metabolism
Hair Follicle/*metabolism
Intercellular Signaling Peptides and Proteins/*metabolism
Membrane Proteins/*metabolism
Alopecia/drug therapy ; Alopecia/genetics ; Dermis/metabolism ; Gene Expression Regulation/genetics ; Humans ; Intracellular Signaling Peptides and Proteins ; Male ; Transcription Factors/metabolism
Contributed Indexing:
Keywords: Androgenetic alopecia (AGA); Forkhead box C1 (FOXC1); Secreted frizzled related protein 1 (SFRP1); Transcriptional regulation
Substance Nomenclature:
0 (FOXC1 protein, human)
0 (Forkhead Transcription Factors)
0 (Intercellular Signaling Peptides and Proteins)
0 (Intracellular Signaling Peptides and Proteins)
0 (Membrane Proteins)
0 (SFRP1 protein, human)
0 (Transcription Factors)
0 (WD repeat containing planar cell polarity effector)
Entry Date(s):
Date Created: 20210509 Date Completed: 20210929 Latest Revision: 20210929
Update Code:
20240105
DOI:
10.1016/j.yexcr.2021.112618
PMID:
33965401
Czasopismo naukowe
Androgenetic alopecia (AGA) is the most common type of hair loss dysfunction. Secreted frizzled related protein 1 (SFRP1) is found to be associated with hair loss, but its role in AGA and the regulation mechanism of its transcription level is unclear. The aim of our study is to explore the expression of SFRP1 in AGA samples and its transcriptional mechanism. Male frontal and occipital scalp hair follicles from AGA patients were collected, and human dermal papilla cells (DPCs) were isolated and cultured. SFRP1 gene was cloned and constructed into recombinant plasmids to perform dual-luciferase reporter assay. Transcription factor binding sites were predicted through the Jaspar website and further confirmed by the chromatin immunoprecipitation (ChIP) assay. Expression of genes in DPCs was determined by immunofluorescence (IF) staining, quantitative real-time PCR (qRT-PCR) and western blotting. Our findings showed that SFRP1 was highly expressed in DPCs of AGA patients. The core promoter region of SFRP1 was from -100 to +50 bp and was found to be positively regulated by forkhead box C1 (FOXC1), a transcription factor related to hair growth, both at mRNA and protein level in DPCs. Our study suggests that FOXC1 plays an important role in regulating SFRP1 transcription, which may provide new insights into the development of therapeutic strategies for the treatment of AGA.
(Copyright © 2021 Elsevier Inc. All rights reserved.)

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