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Tytuł pozycji:

A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M 1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles.

Tytuł:
A novel colorimetric aptasensor for ultrasensitive detection of aflatoxin M 1 based on the combination of CRISPR-Cas12a, rolling circle amplification and catalytic activity of gold nanoparticles.
Autorzy:
Abnous K; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Danesh NM; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Research Institute of Sciences and New Technology, Mashhad, Iran.
Ramezani M; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Alibolandi M; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Nameghi MA; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Zavvar TS; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Taghdisi SM; Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: .
Źródło:
Analytica chimica acta [Anal Chim Acta] 2021 Jun 22; Vol. 1165, pp. 338549. Date of Electronic Publication: 2021 Apr 22.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Amsterdam : Elsevier
Original Publication: Amsterdam.
MeSH Terms:
Biosensing Techniques*
Metal Nanoparticles*
Aflatoxin M1/analysis ; CRISPR-Cas Systems ; Clustered Regularly Interspaced Short Palindromic Repeats ; Colorimetry ; Gold ; Limit of Detection ; Nucleic Acid Amplification Techniques
Contributed Indexing:
Keywords: CRISPR-Cas12a; Catalytic activity; Colorimetric method; Gold nanoparticles; Rolling circle amplification; Ultrasensitive detection
Substance Nomenclature:
6795-23-9 (Aflatoxin M1)
7440-57-5 (Gold)
Entry Date(s):
Date Created: 20210512 Date Completed: 20210519 Latest Revision: 20210519
Update Code:
20240105
DOI:
10.1016/j.aca.2021.338549
PMID:
33975697
Czasopismo naukowe
Colorimetric approaches have received noticeable attention among sensing methods in view of simplicity and watching the color change of sample by the naked eyes. However, developing colorimetric sensing methods which show high sensitivity is still problematic. Herein, based on CRISPR-Cas12a, rolling circle amplification (RCA) and catalytic activity of gold nanoparticles (AuNPs), a colorimetric aptasensor was introduced for highly sensitive detection of aflatoxin M 1 (AFM 1 ). In the presence of AFM 1 , the CRISPR-Cas12a is inactivated and large single-stranded DNA (ssDNA) structures are formed on the surface of AuNPs following the addition of T4 DNA ligase and phi29 DNA polymerase. So, the sample color remains yellow after addition of 4-nitrophenol. However, no huge DNA structure is observed on the surface of AuNPs in the absence of target because of activation of CRISPR-Cas12a and digestion of primer. So, the color of sample switches to colorless. The results indicated that the biosensor had high selectivity toward AFM 1 and the approach achieved a detection limit as low as 0.05 ng/L. In addition, it could sensitively identify AFM 1 in the spiked milk samples. Overall, this approach is highly sensitive and does not require sophisticated equipment. Therefore, it maintains promising potential for other mycotoxins detection in real samples by simply replacing the applied sequences.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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