Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers.

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Abstrakt

Tytuł:: Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers.
Autorzy:: Oliveira-Arbex AP; Universidade Estadual Paulista, Instituto de Biociências, Departmento de Parasitologia, Botucatu, São Paulo, Brazil.; Faculdades Integradas de Bauru, Bauru, São Paulo, Brazil.
David ÉB; Universidade Estadual Paulista, Instituto de Biociências, Departmento de Parasitologia, Botucatu, São Paulo, Brazil.; UNISAGRADO, Departamento de Ciências da Saúde, Bauru, SP, Brazil.
Cacciò SM; Istituto Superiore di Sanità, Dipartimento Malattie Infettive, Parassitarie ed Immunomediate, Rome, Italy.
Fonseca CRBD; Universidade Estadual Paulista, Faculdade de Medicina, Departamento de Pediatria, Botucatu, São Paulo, Brazil.
Martin JG; Universidade Estadual Paulista, Faculdade de Medicina, Departamento de Pediatria, Botucatu, São Paulo, Brazil.
Kurokawa CS; Universidade Estadual Paulista, Faculdade de Medicina, Departamento de Pediatria, Botucatu, São Paulo, Brazil.
Tosini F; Istituto Superiore di Sanità, Dipartimento Malattie Infettive, Parassitarie ed Immunomediate, Rome, Italy.
Souza Neto JA; Universidade Estadual Paulista, Faculdade de Ciências Agronômicas, Departamento de Bioprocessos e Biotecnologia, Botucatu, São Paulo, Brazil.
Guimarães S; Universidade Estadual Paulista, Instituto de Biociências, Departmento de Parasitologia, Botucatu, São Paulo, Brazil.
Źródło:: Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2021 May 07; Vol. 63, pp. e39. Date of Electronic Publication: 2021 May 07 (Print Publication: 2021).
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo
MeSH Terms:: Dientamoebiasis*/diagnosis
Dientamoebiasis*/epidemiology
Intestinal Diseases, Parasitic*
Brazil/epidemiology ; Child ; Child, Preschool ; Dientamoeba/genetics ; Feces ; Humans ; Prevalence
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Entry Date(s):: Date Created: 20210512 Date Completed: 20210513 Latest Revision: 20210515
Update Code:: 20240105
PubMed Central ID:: PMC8112823
DOI:: 10.1590/S1678-9946202163039
PMID:: 33978095
: Czasopismo naukowe

In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State, Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and, (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal-oral route.

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