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Tytuł:
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Use of photoacoustic imaging to determine the effects of aging on lower extremity lymphatic vessel function.
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Autorzy:
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Suzuki Y; Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan. Electronic address: .
Kajita H; Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan.
Oh A; Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan.
Takemaru M; Department of Plastic and Reconstructive Surgery, Yokohama Municipal Citizen Hospital, Kanagawa, Japan.
Sakuma H; Department of Plastic and Reconstructive Surgery, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan.
Tsuji T; Department of Rehabilitation Medicine, Keio University School of Medicine, Tokyo, Japan.
Imanishi N; Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
Aiso S; Department of Anatomy, Keio University School of Medicine, Tokyo, Japan; Luxonus Inc, Kawasaki, Japan.
Kishi K; Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan.
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Źródło:
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Journal of vascular surgery. Venous and lymphatic disorders [J Vasc Surg Venous Lymphat Disord] 2022 Jan; Vol. 10 (1), pp. 125-130. Date of Electronic Publication: 2021 May 18.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: New York, NY : Elsevier Inc., c2013-
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MeSH Terms:
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Photoacoustic Techniques*
Aging/*physiology
Lymphatic System/*diagnostic imaging
Lymphatic System/*physiology
Adult ; Female ; Humans ; Lower Extremity ; Male ; Middle Aged
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Contributed Indexing:
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Keywords: Aging; Lymphatic vessel; Photoacoustic imaging; Photoacoustic lymphangiography
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Entry Date(s):
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Date Created: 20210521 Date Completed: 20220303 Latest Revision: 20220303
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Update Code:
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20240105
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DOI:
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10.1016/j.jvsv.2021.05.001
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PMID:
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34020108
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Objective: Aging is one of the causes of primary lymphedema. However, the effects of aging on the lymphatic system are still not completely understood. We investigated the effects of aging on the lymphatic vessels in the lower extremities of healthy volunteers using photoacoustic imaging.
Methods: Healthy volunteers who underwent photoacoustic lymphangiography between March 2018 and January 2019 were enrolled. To visualize lymphatics, indocyanine green (ICG, 5.0 mg/mL) was injected subcutaneously into the first and fourth web spaces of the foot and under the lateral malleolus. Subsequently, near-infrared fluorescence lymphography was performed to confirm good ICG flow, and photoacoustic lymphangiography was performed on the medial side of the lower leg. Ti sapphire laser irradiation at 797 and 835 nm, the optimal wavelengths for visualizing ICG and blood, was applied. The number of lymphatic vessels shown at areas 10 cm (L10) and 20 cm (L20) cranially from the internal malleolus was counted.
Results: Nineteen healthy volunteers (4 males and 15 females) were enrolled in the study. Their mean age was 42.9 ± 12.8 years. One volunteer was bilaterally imaged; 15 left lower limbs and 5 right lower limbs were imaged. The number of lymphatic vessels visualized increased with age. There were strong positive correlations between age and L10 (R = 0.729, P < .001) and between age and L20 (R = 0.570, P = .009).
Conclusions: Photoacoustic imaging indicates that the number of lymphatic vessels increases with age. Lymphatic stasis resulted in visualization of not only normal drainage pathways but also nonfunctional lymphatic pathways.
(Copyright © 2021 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.)
