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Tytuł:
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Hyaluronan (HA) Immobilized on Surfaces via Self-Assembled Monolayers of HA-Binding Peptide Modulates Endothelial Cell Spreading and Migration through Focal Adhesion.
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Autorzy:
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Pang X; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
Li W; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
Chang L; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
Gautrot JE; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
Wang W; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
Azevedo HS; School of Engineering and Materials Science, Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.
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Źródło:
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ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Jun 09; Vol. 13 (22), pp. 25792-25804. Date of Electronic Publication: 2021 May 26.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, D.C. : American Chemical Society
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MeSH Terms:
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Cell Movement*
Extracellular Matrix/*metabolism
Focal Adhesions/*metabolism
Human Umbilical Vein Endothelial Cells/*physiology
Hyaluronan Receptors/*metabolism
Hyaluronic Acid/*metabolism
Peptide Fragments/*metabolism
Human Umbilical Vein Endothelial Cells/cytology ; Humans ; Hyaluronic Acid/chemistry ; Sulfhydryl Compounds/chemistry ; Surface Properties
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Contributed Indexing:
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Keywords: endothelial cells; focal adhesion; hyaluronan; model surfaces; peptides; self-assembled monolayers
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Substance Nomenclature:
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0 (CD44 protein, human)
0 (Hyaluronan Receptors)
0 (Peptide Fragments)
0 (Sulfhydryl Compounds)
42GO2PA46L (n-octanethiol)
9004-61-9 (Hyaluronic Acid)
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Entry Date(s):
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Date Created: 20210526 Date Completed: 20210728 Latest Revision: 20210728
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Update Code:
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20240105
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DOI:
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10.1021/acsami.1c05574
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PMID:
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34037376
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The extracellular matrix (ECM) modulates a multitude of cell functions, and this regulation is provided by key ECM components forming a complex network. Hyaluronic acid (HA) is an abundant component of the ECM that binds to proteins and influences various activities of endothelial cells (ECs). Although the effect of soluble HA on cell spreading has been studied, the impact of peptide-bound HA has not yet been investigated in great detail. We aim to comprehensively study the roles of immobilized HA on the regulation of EC behavior compared to the more conventional use of soluble HA. A 2D model surface formed by self-assembled monolayers (SAMs) of a HA-binding peptide (Pep-1) is used as an anchor for HA immobilization. Mixed SAMs, consisting of thiolated Pep-1 and 1-octanethiol, are prepared and characterized by using ellipsometry and contact angle measurement. Full density Pep-1 SAMs are more hydrophilic and bind more HA than mixed SAMs. Cell spreading and migration are enhanced by immobilized low molecular weight (LMW) HA, which also facilitates cell alignment and elongation under laminar flow conditions and potentially drives directional migration. This effect is not mediated by the expression of CD44, and immobilized LMW HA is found to accelerate the assembly of focal adhesions. Such biomimetic surfaces provide new insights into the role of HA in regulating the spreading and phenotype of endothelial cells.
