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Tytuł:
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Bio-inspired Ag nanovilli-based sandwich-type SERS aptasensor for ultrasensitive and selective detection of 25-hydroxy vitamin D 3 .
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Autorzy:
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Kim W; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Park J; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Kim W; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Jo S; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea; Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea.
Kim M; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Kim C; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Park H; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea.
Bang D; College of AI Convergence, Chonnam National University, Gwangju, 61186, South Korea; Korea Institute of Medical Microrobotics, Gwangju, 61011, South Korea.
Lee W; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea; Department of Electrical Engineering, Korea National University of Transportation, Chungju, 27469, South Korea. Electronic address: .
Park J; Department of Control and Instrumentation Engineering, Korea University, Sejong, 30019, South Korea. Electronic address: .
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Źródło:
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Biosensors & bioelectronics [Biosens Bioelectron] 2021 Sep 15; Vol. 188, pp. 113341. Date of Electronic Publication: 2021 May 14.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Elsevier Advanced Technology
Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
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MeSH Terms:
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Aptamers, Nucleotide*
Biosensing Techniques*
COVID-19*
Metal Nanoparticles*
Calcifediol ; Gold ; Humans ; Reproducibility of Results ; SARS-CoV-2 ; Silver ; Spectrum Analysis, Raman
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Contributed Indexing:
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Keywords: 25-Hydroxy vitamin D(3); Ag nanovilli; Aptasensor; Bio-inspired; Surface enhanced Raman spectroscopy
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Substance Nomenclature:
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0 (Aptamers, Nucleotide)
3M4G523W1G (Silver)
7440-57-5 (Gold)
P6YZ13C99Q (Calcifediol)
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Entry Date(s):
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Date Created: 20210527 Date Completed: 20210611 Latest Revision: 20210611
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Update Code:
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20240105
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DOI:
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10.1016/j.bios.2021.113341
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PMID:
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34044348
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Vitamin D has been identified as an essential biomarker for various diseases such as rheumatoid arthritis, cancer, and cardiovascular diseases. Recently, many reports have demonstrated a potential link between vitamin D and systemic infections, including coronavirus disease 2019. The villi of the small intestine increase the surface area of the intestinal walls, demonstrating exceptionally efficient absorption of nutrients in the lumen and adding digestive secretions. In this study, based on the villi structure, we developed a bio-inspired silver nanovilli-based sandwich-type surface enhanced Raman scattering aptasensor for the ultrasensitive and selective detection of 25-hydroxy vitamin D 3 . The densely packed nanovilli structure enhanced the Raman signal, forming hotspots owing to its large surface area. Using experiments and electromagnetic simulations, we optimized the nanovilli structure as a SERS sensor. The sandwich-type aptasensor was designed using an aptamer and 4-Phenyl-1,2,4-triazoline-3,5-dione-methylene blue complex. The nanovilli-based aptasensor could sensitively detect various concentrations of 25-hydroxy vitamin D 3 , ranging from those found in deficient to excess conditions. The detection limit of the nanovilli-based sandwich-type aptasensor for 25-hydroxy vitamin D 3 was 0.001 ng/mL, which is much lower than the deficiency concentration, and was detectable even in the human serum. In addition, our proposed sensor exhibited good repeatability (17.76%) and reproducibility (7.47%). Moreover, the nanovilli-based sandwich-type SERS aptasensor could selectively distinguish 25-hydroxy vitamin D 3 from other vitamins. The silver nanovilli-based sandwich-type surface enhanced Raman scattering aptasensor opens a new avenue for the development of a bio-inspired vitamin-sensing platform.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
