Development of immunofluorescent diagnostics for the determination of IgM and IgG antibodies to herpes simplex virus types 1 and 2.

Szczegóły
Abstrakt

Tytuł:: Development of immunofluorescent diagnostics for the determination of IgM and IgG antibodies to herpes simplex virus types 1 and 2.
Autorzy:: Shershneva NN; EKOlab.
Mardanly SS; EKOlab.; National Research Center of Epidemiology and Microbiology named after honorary academician N.F. Gamalei of the Ministry of Health of the Russian Federation.
Klenyaev IN; EKOlab.
Samosadova PV; EKOlab.
Transliterated Title:: Разработка иммунофлюоресцентных диагностикумов для выявления антител к герпесвирусам 1и 2 типов.
Źródło:: Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2021 May 23; Vol. 66 (5), pp. 285-290.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Moskva : "Meditsina", 1992-
MeSH Terms:: Herpes Simplex*/diagnosis
Herpesvirus 1, Human*
Antibodies, Viral ; Enzyme-Linked Immunosorbent Assay ; Herpesvirus 2, Human ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Russia
References:: Chayavichitsilp P., Buckwalter J.V., Krakowski A.C., Sheila F. Friedlander. HerpesSimplex. Pediatrics in Review. 2009; 30(4): 119-29.
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Mardanly S. G. Herpessimplex infections. [Gerpesvirusnye infektsii]. Orekhovo-Zuevo: GGTU; 2017. (in Russian).
Mardanly S. G, Arsen’eva V.A., Mardanly S.S., Rotanov S.V.. The prevalence of human herpes viruses among contingents of different ages. Zhurnal mikrobiologii, epidemiologii i immunobiologii. 2019; 2: 50. (in Russian).
Panchenko L.A., Kirichenko I.I., Khodak L.A. The causative agents of herpesvirus infections and the most important clinical manifestations in humans. Provizor. 1999; 10: 24-8.
Bogatyreva L.N. Congenital infection caused by the herpes simplex virus (Herpes simplex): etiology, pathogenesis, clinical picture, diagnosis, treatment, prevention. Meditsina. Sotsiologiya. Filosofiya. Prikladnye issledovaniya. 2019; 1: 46. (in Russian).
Mardanly S.G., Kirpichnikova G.I., Neverov V.A., Amelina E.A.. Herpesvirus infections: features of pathogenesis, diagnosis, treatment. Meditsinskiy alfavit. 2012; 4 (20): 48-50. (in Russian).
Mardanly S. G., Kirpichnikova G. I., Neverov V. A. Herpes infection (herpes simplex). Etiology, epidemiology, pathogenesis, clinic, laboratory diagnostics, treatment. Elektrogorsk: ZAO «EKOlab»; 2011. (in Russian).
Rybalkina T.N., Karazhas N.V., Savinkov P.A., Bosh’yan R.E., Lysenkova M.Yu., Kornienko M.N. et al. The importance of herpes viruses in the etiology of a number of infectious and somatic diseases in children. Detskie infektsii. 2017; 16(3):10—9. (in Russian).
Pavlyuk A.S. Methods of laboratory diagnosis of diseases caused by the herpes simplex virus. Zabolevaniya, peredavaemye polovym putem. 1994; 3: 3-7. (in Russian).
Mardanly S.G., Simonova E.G., Simonov V.V. Infections of the TORCH group: clinical laboratory diagnostics, epidemiological surveillance and control. [Infekcii ToRCH-gruppy: klinicheskaya laboratornaya diagnostika, epidemiologicheskij nadzor i kontrol’]. Orekhovo-Zuevo:Gosudarstvennyi gumanitarno-tekhnologicheskiy universitet; 2018. (in Russian).
Mardanly S.G., Avdonina A.S., Mardanly S.S., N. N. Shershneva N.N. Development of a set of reagents for the detection of class G antibodies to individual antigens of herpes simplex viruses of the first and second types by immune blotting in the Western-Line-Blot format. Meditsinskiy alfavit. 2017; 38 (4): 33-40. (in Russian).
Mardanly S.G., Tomashevskaya N.A., Muhina A.I, Gvil’dis I.YU., Kirillova L.D, Hozhainova M.P.. Experience in using a complex of test systems for diagnosing infections of the TORCH group. Epidemiologiya i infektsionnye bolezni. Aktual’nye voprosy. 2014; 5: 65-9. (in Russian).
Michael R. Lewis, Jim Y. Kao, Anne-Line J. Anderson, John E. Shively and Andrew Raubitschek. An improved method for conjugating monoclonal antibodies with N-hydroxysulfosuccinimidyl DOTA. Bioconjugate chemistry. 2001; 12 (2): 320-4.
Mardanly S.G. Immunoassay test systems of CJSC «EKOlab» for the diagnosis of herpes simplex. Klinicheskaya laboratornaya diagnostika. 2008; 2: 35-8. (in Russian).
Alekseev Yu.D., Savenkova E.N. Types of specific fluorescence in the reaction of immunofluorescence (IFA). Problemy ekspertizy v meditsine. 2013; 13 (2); 25-6. (in Russian).
Mardanly S.G. Tasks and prospects for improving the clinical laboratory diagnosis of infections of the TORCH group. Vestnik sluzhby krovi Rossii. 2013; 2: 54-60. (in Russian).
Contributed Indexing:: Keywords: HSV-1; HSV-2; development; diagnostics; immunofluorescence assay
Substance Nomenclature:: 0 (Antibodies, Viral)
0 (Immunoglobulin G)
0 (Immunoglobulin M)
Entry Date(s):: Date Created: 20210528 Date Completed: 20210531 Latest Revision: 20210531
Update Code:: 20240105
DOI:: 10.51620/0869-2084-2021-66-5-285-290
PMID:: 34047514
: Czasopismo naukowe

The Russian kits «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen» have been developed for the determination of antibodies M and G to herpes simplex virus types 1 and 2 by the immunofluorescence reaction. The kits were used to examine the positive and negative standard «EKOlab» panels sera and showed 100% sensitivity and specificity of the developed tests. 125 samples of blood serum from people with clinical diagnoses such as herpetic, cytomegalovirus infections, pyelonephritis, conjunctivitis and central nervous system damage were tested in parallel with using the enzyme-linked immunosorbent assay systems from different manufacturers and the developed tests «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen». A high degree of matching of results with comparison sets was observed in examined samples. The developed diagnostics can be successfully used in clinical practice both for screening and for verification of results of the diagnosis of herpesvirus infections caused by herpes simplex virus types 1 and 2.
Competing Interests: The authors declare no conflict of interest.

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