Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins.

Tytuł:: Microfluidic ion stripper for removal of trifluoroacetic acid from mobile phases used in HILIC-MS of intact proteins.
Autorzy:: Wouters S; Department of Chemical Engineering, Vrije Universiteit Brussel (VUB), 1050, Brussels, Belgium.
Eeltink S; Department of Chemical Engineering, Vrije Universiteit Brussel (VUB), 1050, Brussels, Belgium. .
Haselberg R; Center for Analytical Sciences Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.; Division of BioAnalytical Chemistry, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.
Somsen GW; Center for Analytical Sciences Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.; Division of BioAnalytical Chemistry, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.
Gargano AFG; Center for Analytical Sciences Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands. .; Division of BioAnalytical Chemistry, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands. .
Źródło:: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Jul; Vol. 413 (17), pp. 4379-4386. Date of Electronic Publication: 2021 May 28.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Heidelberg : Springer-Verlag, 2002-
MeSH Terms:: Lab-On-A-Chip Devices*
Proteins/*analysis
Trifluoroacetic Acid/*isolation & purification
Animals ; Cattle ; Chickens ; Chromatography, Liquid ; Equipment Design ; Horses ; Hydrophobic and Hydrophilic Interactions ; Spectrometry, Mass, Electrospray Ionization
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Grant Information:: 722.015.009 NWO; G033018N Fonds Wetenschappelijk Onderzoek
Contributed Indexing:: Keywords: Intact protein analysis; LC ion-pairing; LC-MS; MS ion suppression; Microfluidic chips; TFA
Substance Nomenclature:: 0 (Proteins)
E5R8Z4G708 (Trifluoroacetic Acid)
Entry Date(s):: Date Created: 20210529 Date Completed: 20210910 Latest Revision: 20210910
Update Code:: 20240105
PubMed Central ID:: PMC8245364
DOI:: 10.1007/s00216-021-03414-4
PMID:: 34050389
: Czasopismo naukowe

Pełny tekst

Trifluoroacetic acid (TFA) is commonly used as mobile phase additive to improve retention and peak shape characteristics in hydrophilic interaction liquid chromatography (HILIC) of intact proteins. However, when using electrospray ionization-mass spectrometry (ESI-MS) detection, TFA may cause ionization suppression and adduct formation, leading to reduced analyte sensitivity. To address this, we describe a membrane-based microfluidic chip with multiple parallel channels for the selective post-column removal of TFA anions from HILIC. An anion-exchange membrane was used to physically separate the column effluent from a stripper flow solution comprising acetonitrile, formic acid, and propionic acid. The exchange of ions allowed the post-column removal of TFA used during HILIC separation of model proteins. The multichannel design of the device allows the use of flow rates of 0.2 mL/min without the need for a flow splitter, using mobile phases containing 0.1% TFA (13 mM). Separation selectivity and efficiency were maintained (with minor band broadening effects) while increasing the signal intensity and peak areas by improving ionization and reducing TFA adduct formation.

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