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Tytuł pozycji:

Expression of long-chain polyunsaturated fatty acids biosynthesis genes during the early life-cycle stages of the tropical gar Atractosteus tropicus.

Tytuł:
Expression of long-chain polyunsaturated fatty acids biosynthesis genes during the early life-cycle stages of the tropical gar Atractosteus tropicus.
Autorzy:
De la Cruz-Alvarado FJ; Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, C.P.86139 Villahermosa, Tabasco, Mexico.
Álvarez-González CA; Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, C.P.86139 Villahermosa, Tabasco, Mexico.
Llera-Herrera R; Instituto de Ciencias del Mar y Limnología - Unidad Académica Mazatlán, Universidad Nacional Autónoma de México, Joel Montes Camarena S/N, PO Box 811, Mazatlán, Sinaloa, Mexico.
Monroig Ó; Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
Kabeya N; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Rodríguez-Morales S; Facultad de Química, Universidad Nacional Autónoma de México, Unidad de Química-Sisal, Puerto de Abrigo s/n, Sisal, Yucatán, Mexico.
Concha-Frias B; División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Nacajuca-Jalpa de Méndez R/a Rivera Alta, C.P. 86200 Jalpa de Méndez, Tabasco, Mexico.
Guerrero-Zárate R; Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, C.P.86139 Villahermosa, Tabasco, Mexico.
Jiménez-Martínez LD; División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Nacajuca-Jalpa de Méndez R/a Rivera Alta, C.P. 86200 Jalpa de Méndez, Tabasco, Mexico. Electronic address: .
Peña-Marín ES; Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, C.P.86139 Villahermosa, Tabasco, Mexico; Consejo Nacional de Ciencia y Tecnología, CDMX, Mexico. Electronic address: .
Źródło:
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2021 Oct-Dec; Vol. 256, pp. 110628. Date of Electronic Publication: 2021 May 28.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Oxford : Pergamon
Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994-
MeSH Terms:
Gene Expression Regulation, Developmental*
Lipogenesis*
Fatty Acid Desaturases/*genetics
Fatty Acid Elongases/*genetics
Fatty Acids, Unsaturated/*biosynthesis
Fish Proteins/*metabolism
Fishes/*metabolism
Animals ; Fish Proteins/genetics ; Fishes/genetics ; Fishes/growth & development ; Transcriptome
Contributed Indexing:
Keywords: Desaturase; Early development; Elongase; LC-PUFA biosynthesis; Lepisosteidae
Substance Nomenclature:
0 (Fatty Acids, Unsaturated)
0 (Fish Proteins)
EC 1.14.19.- (Fatty Acid Desaturases)
EC 2.3.1.- (Fatty Acid Elongases)
Entry Date(s):
Date Created: 20210530 Date Completed: 20210924 Latest Revision: 20210924
Update Code:
20240105
DOI:
10.1016/j.cbpb.2021.110628
PMID:
34052410
Czasopismo naukowe
Long-chain (≥C 20 ) polyunsaturated fatty acids (LC-PUFA), including eicosapentaenoic acid (EPA, 20:5n-3), arachidonic acid (ARA, 20:4n-6) and docosahexaenoic acid (DHA, 22:6n-3), are essential in multiple physiological processes, especially during early development of vertebrates. LC-PUFA biosynthesis is achieved by two key families of enzymes, fatty acyl desaturases (Fads) and elongation of very long-chain fatty acid (Elovl). The present study determined the expression patterns of genes encoding desaturases (fads1 and fads2) and elongases (elovl2 and elovl5) involved in the LC-PUFA biosynthesis during early life-stages of the tropical gar Atractosteus tropicus. We further analyzed the fatty acid profiles during early development of A. tropicus to evaluate the impact of Fads and Elovl enzymatic activities. Specific oligonucleotides were designed from A. tropicus transcriptome to perform qPCR (quantitative polymerase chain reaction) on embryonic and larval stages, along with several organs (intestine, white muscle, brain, liver, heart, mesenteric adipose, kidney, gill, swim bladder, stomach, and spleen) collected from juvenile specimens. Fatty acid content of feeds and embryonic and larval stages were analyzed. Results show that fads1, fads2, elovl2 and elovl5 expression was detected from embryonic stages with expression peaks from day 15 post hatching, which could be related to transcriptional and dietary factors. Moreover, fads1, fads2 and elovl2 showed a higher expression in intestine, while elovl5 showed a higher expression in liver, suggesting that the tropical gar activates its LC-PUFA biosynthetic machinery to produce ARA, EPA and DHA to satisfy physiological demands at crucial developmental milestones during early development.
(Copyright © 2021 Elsevier Inc. All rights reserved.)

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