Cytogenetic markers using single-sequence probes reveal chromosomal locations of tandemly repetitive genes in scleractinian coral Acropora pruinosa.

Tytuł:: Cytogenetic markers using single-sequence probes reveal chromosomal locations of tandemly repetitive genes in scleractinian coral Acropora pruinosa.
Autorzy:: Vacarizas J; Kuroshio Science Program, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan. .
Taguchi T; Department of Nutrition, Faculty of Health Sciences, Kochi Gakuen University, Kochi, Kochi, Japan.
Mezaki T; Kuroshio Biological Research Foundation, Otsuki, Kochi, Japan.
Okumura M; Sea Nature Museum Marine Jam, Kaiyo, Tokushima, Japan.
Kawakami R; Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan.
Ito M; Faculty of Agriculture and Marine Science, Kochi University, Nankoku, Kochi, Japan.
Kubota S; Kuroshio Science Program, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan.
Źródło:: Scientific reports [Sci Rep] 2021 May 31; Vol. 11 (1), pp. 11326. Date of Electronic Publication: 2021 May 31.
Typ publikacji:: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Chromosomes*
Cytogenetic Analysis*
Molecular Probe Techniques*
Anthozoa/*genetics
Animals ; Histones/genetics ; RNA, Ribosomal, 5S/genetics
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Substance Nomenclature:: 0 (Histones)
0 (RNA, Ribosomal, 5S)
Entry Date(s):: Date Created: 20210601 Date Completed: 20211119 Latest Revision: 20230202
Update Code:: 20240105
PubMed Central ID:: PMC8167085
DOI:: 10.1038/s41598-021-90580-1
PMID:: 34059722
: Czasopismo naukowe

Pełny tekst

The short and similar sized chromosomes of Acropora pose a challenge for karyotyping. Conventional methods, such as staining of heterochromatic regions, provide unclear banding patterns that hamper identification of such chromosomes. In this study, we used short single-sequence probes from tandemly repetitive 5S ribosomal RNA (rRNA) and core histone coding sequences to identify specific chromosomes of Acropora pruinosa. Both the probes produced intense signals in fluorescence in situ hybridization, which distinguished chromosome pairs. The locus of the 5S rDNA probe was on chromosome 5, whereas that of core histone probe was on chromosome 8. The sequence of the 5S rDNA probe was composed largely of U1 and U2 spliceosomal small nuclear RNA (snRNA) genes and their interspacers, flanked by short sequences of the 5S rDNA. This is the first report of a tandemly repetitive linkage of snRNA and 5S rDNA sequences in Cnidaria. Based on the constructed tentative karyogram and whole genome hybridization, the longest chromosome pair (chromosome 1) was heteromorphic. The probes also hybridized effectively with chromosomes of other Acropora species and population, revealing an additional core histone gene locus. We demonstrated the applicability of short-sequence probes as chromosomal markers with potential for use across populations and species of Acropora.

Erratum in: Sci Rep. 2021 Aug 12;11(1):16758. (PMID: 34385573)

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