Highly Sensitive Colorimetric Assay of Cortisol Using Cortisol Antibody and Aptamer Sandwich Assay.

Szczegóły
Abstrakt

Tytuł:: Highly Sensitive Colorimetric Assay of Cortisol Using Cortisol Antibody and Aptamer Sandwich Assay.
Autorzy:: Kim Y; Department of Chemical Engineering, Myongji University, Yongin-si 17058, Gyeonggi-do, Korea.
Yang J; Department of Chemical Engineering, Myongji University, Yongin-si 17058, Gyeonggi-do, Korea.
Hur H; Department of Chemical Engineering, Myongji University, Yongin-si 17058, Gyeonggi-do, Korea.
Oh S; Department of Chemical Engineering, Myongji University, Yongin-si 17058, Gyeonggi-do, Korea.
Lee HH; Department of Chemical Engineering, Myongji University, Yongin-si 17058, Gyeonggi-do, Korea.
Źródło:: Biosensors [Biosensors (Basel)] 2021 May 20; Vol. 11 (5). Date of Electronic Publication: 2021 May 20.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI Pub.
MeSH Terms:: Aptamers, Nucleotide*
Biosensing Techniques*
Colorimetry*
Hydrocortisone/*chemistry
Antibodies ; Gold ; Limit of Detection ; Thrombin
References:: Science. 1997 Aug 22;277(5329):1078-81. (PMID: 9262471)
Anal Bioanal Chem. 2014 Jul;406(19):4637-47. (PMID: 24880870)
Annu Rev Anal Chem (Palo Alto Calif). 2009;2:241-64. (PMID: 20636061)
Biosens Bioelectron. 2012 Apr 15;34(1):1-11. (PMID: 22326894)
Sensors (Basel). 2017 May 22;17(5):. (PMID: 28531146)
Biosens Bioelectron. 2020 Jul 1;159:112186. (PMID: 32364939)
Anal Chem. 2014 Jan 7;86(1):814-9. (PMID: 24328254)
Biosens Bioelectron. 2017 Dec 15;98:473-477. (PMID: 28728007)
ACS Omega. 2020 Dec 17;5(51):32890-32898. (PMID: 33403250)
Biosens Bioelectron. 2013 Mar 15;41:186-91. (PMID: 22939507)
Nat Biotechnol. 2004 Jul;22(7):883-7. (PMID: 15170215)
Nat Protoc. 2006;1(1):246-52. (PMID: 17406240)
Grant Information:: 2020R1F1A1073708 National Research Foundation of Korea; 2019002790002 Korea Environment Industry & Technology Institute (KEITI)
Contributed Indexing:: Keywords: antibody aptamer sandwich; c-Mab; c-SBA; colorimetric assay; cortisol
Substance Nomenclature:: 0 (Antibodies)
0 (Aptamers, Nucleotide)
7440-57-5 (Gold)
EC 3.4.21.5 (Thrombin)
WI4X0X7BPJ (Hydrocortisone)
Entry Date(s):: Date Created: 20210602 Date Completed: 20210604 Latest Revision: 20210615
Update Code:: 20240104
PubMed Central ID:: PMC8160784
DOI:: 10.3390/bios11050163
PMID:: 34065214
: Czasopismo naukowe

Full Text Finder

In this study, cortisol, which is a key stress hormone, could be detected sensitively via the colorimetric assay of a polycarbonate (PC) and glass substrate by the sandwich assay of cortisol monoclonal antibody (c-Mab) and cortisol specific binding aptamer (c-SBA). A highly sensitive change in colorimetry with a limit of detection (LOD) of cortisol of 100 fM could be attained on the optically transparent substrate using the antibody aptamer sandwich (AAS) assay by corresponding stacks of 5 nm gold nanoparticles (Au NPs). The Au NPs were conjugated by the c-SBA and the c-Mab was tethered on the PC and glass substrates. For the AAS method, a simple UV-Vis spectrophotometer was adopted to quantify the cortisol concentrations at an absorbance wavelength of 520 nm. Therefore, this study demonstrates the versatility of the AAS method to measure very low concentrations of cortisol in diagnostic applications.

Informacja