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Tytuł pozycji:

TILLING-by-Sequencing + Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content.

Tytuł:
TILLING-by-Sequencing Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content.
Autorzy:
Lakhssassi N; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Lopes-Caitar VS; Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA.
Knizia D; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Cullen MA; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Badad O; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
El Baze A; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Zhou Z; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Embaby MG; Department of Animal Science, Food, and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA.
Meksem J; Trinity College of Arts and Sciences, Duke University, Durham, NC 27708, USA.
Lakhssassi A; Faculty of Sciences and Technologies, University of Lorraine, 54506 Nancy, France.
Chen P; Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA.
AbuGhazaleh A; Department of Animal Science, Food, and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA.
Vuong TD; Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA.
Nguyen HT; Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA.
Hewezi T; Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA.
Meksem K; Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
Źródło:
Cells [Cells] 2021 May 19; Vol. 10 (5). Date of Electronic Publication: 2021 May 19.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: Basel, Switzerland : MDPI
MeSH Terms:
DNA Mutational Analysis*
Mutagenesis, Site-Directed*
Fatty Acid Desaturases/*metabolism
Oleic Acid/*metabolism
Plant Proteins/*metabolism
Plants, Genetically Modified/*enzymology
Seeds/*enzymology
Glycine max/*enzymology
Fatty Acid Desaturases/genetics ; Gene Expression Regulation, Plant ; Genotype ; High-Throughput Nucleotide Sequencing ; Mutation ; Phenotype ; Phylogeny ; Plant Proteins/genetics ; Plants, Genetically Modified/genetics ; Seeds/genetics ; Glycine max/genetics
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Grant Information:
2020-162-0127 United Soybean Board
Contributed Indexing:
Keywords: GmFAD2-2 subfamily; TILLING-by-Sequencing+; alternative fatty acid pathway; high oleic acid; neofunctionalization; subfunctionalization
Substance Nomenclature:
0 (Plant Proteins)
2UMI9U37CP (Oleic Acid)
EC 1.14.19.- (Fatty Acid Desaturases)
EC 1.14.99.- (delta-12 fatty acid desaturase)
Entry Date(s):
Date Created: 20210602 Date Completed: 20211025 Latest Revision: 20231213
Update Code:
20240104
PubMed Central ID:
PMC8158723
DOI:
10.3390/cells10051245
PMID:
34069320
Czasopismo naukowe
Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B . However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase ( GmFAD2-1 ) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A , GmFAD2-2B , GmFAD2-2C , GmFAD2-2D , and GmFAD2-2E . Segmental duplication of GmFAD2-1A / GmFAD2-1B , GmFAD2-2A/GmFAD2-2C , GmFAD2-2A/GmFAD2-2D , and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A , GmFAD2-2B , GmFAD2-2C , GmFAD2-2D , and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A , GmFAD2-2B , GmFAD2-2C , GmFAD2-2D , and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

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