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Tytuł pozycji:

Generation of GCaMP6s-Expressing Zebrafish to Monitor Spatiotemporal Dynamics of Calcium Signaling Elicited by Heat Stress.

Tytuł:
Generation of GCaMP6s-Expressing Zebrafish to Monitor Spatiotemporal Dynamics of Calcium Signaling Elicited by Heat Stress.
Autorzy:
Li F; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Long Y; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Xie J; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Ren J; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Zhou T; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Song G; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Li Q; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Cui Z; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.
Źródło:
International journal of molecular sciences [Int J Mol Sci] 2021 May 24; Vol. 22 (11). Date of Electronic Publication: 2021 May 24.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:
Calcium Signaling*/drug effects
Calcium Signaling*/genetics
Calcium Signaling*/physiology
Calcium/*metabolism
Calmodulin/*metabolism
Green Fluorescent Proteins/*metabolism
Heat-Shock Response/*genetics
Zebrafish/*genetics
Animals ; Animals, Genetically Modified ; Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & inhibitors ; Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism ; Calmodulin/genetics ; Capsaicin/analogs & derivatives ; Capsaicin/pharmacology ; Green Fluorescent Proteins/genetics ; Heat Shock Transcription Factors/antagonists & inhibitors ; Heat Shock Transcription Factors/metabolism ; Heat-Shock Proteins/genetics ; Heat-Shock Proteins/metabolism ; Heat-Shock Response/physiology ; In Situ Hybridization ; Lanthanum/pharmacology ; Microscopy, Fluorescence ; Spatio-Temporal Analysis ; Zebrafish/growth & development ; Zebrafish/metabolism ; Zebrafish Proteins/antagonists & inhibitors ; Zebrafish Proteins/metabolism
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Grant Information:
2020B0202080005 Key-Area Research and Development Program of Guangdong Province; 2021GDASYL-20210102003 Special Fund Project for Guangdong Academy of Sciences to Build Domestic First-class Research institutions; 31772836 National Natural Science Foundation of China; 31721005 National Natural Science Foundation of China; 2016305 Youth Innovation Promotion Association of Chinese Academy of Sciences; 2019FBZ05 State Key Laboratory of Freshwater Ecology and Biotechnology
Contributed Indexing:
Keywords: GCaMP6s; calcium signaling; heat shock response; heat stress; transgenic zebrafish
Substance Nomenclature:
0 (Calmodulin)
0 (Heat Shock Transcription Factors)
0 (Heat-Shock Proteins)
0 (Zebrafish Proteins)
0 (hsf1 protein, zebrafish)
04M8624OXV (lanthanum chloride)
147336-22-9 (Green Fluorescent Proteins)
6I3K30563S (Lanthanum)
EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
LFW48MY844 (capsazepine)
S07O44R1ZM (Capsaicin)
SY7Q814VUP (Calcium)
Entry Date(s):
Date Created: 20210602 Date Completed: 20210624 Latest Revision: 20210624
Update Code:
20240104
PubMed Central ID:
PMC8197303
DOI:
10.3390/ijms22115551
PMID:
34074030
Czasopismo naukowe
The ability of organisms to quickly sense and transduce signals of environmental stresses is critical for their survival. Ca 2+ is a versatile intracellular messenger involved in sensing a wide variety of stresses and regulating the subsequent cellular responses. So far, our understanding for calcium signaling was mostly obtained from ex vivo tissues and cultured cell lines, and the in vivo spatiotemporal dynamics of stress-triggered calcium signaling in a vertebrate remains to be characterized. Here, we describe the generation and characterization of a transgenic zebrafish line with ubiquitous expression of GCaMP6s, a genetically encoded calcium indicator (GECI). We developed a method to investigate the spatiotemporal patterns of Ca 2+ events induced by heat stress. Exposure to heat stress elicited immediate and transient calcium signaling in developing zebrafish. Cells extensively distributed in the integument of the head and body trunk were the first batch of responders and different cell populations demonstrated distinct response patterns upon heat stress. Activity of the heat stress-induced calcium signaling peaked at 30 s and swiftly decreased to near the basal level at 120 s after the beginning of exposure. Inhibition of the heat-induced calcium signaling by LaCl 3 and capsazepine and treatment with the inhibitors for CaMKII (Ca² 2 /calmodulin-dependent protein kinase II) and HSF1 (Heat shock factor 1) all significantly depressed the enhanced heat shock response (HSR). Together, we delineated the spatiotemporal dynamics of heat-induced calcium signaling and confirmed functions of the Ca 2+ -CaMKII-HSF1 pathway in regulating the HSR in zebrafish.
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