High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.

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Tytuł:: High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.
Autorzy:: Lavier J; Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), Lausanne, Switzerland.; Institute of Sport Sciences, University of Lausanne, Lausanne, Switzerland.
Beaumann M; Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Menétrey S; Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Bouzourène K; Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Rosenblatt-Velin N; Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Pialoux V; Inter-University Laboratory of Human Movement Biology EA7424, University of Lyon, Villeurbanne, France.
Mazzolai L; Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Peyter AC; Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Pellegrin M; Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), Lausanne, Switzerland.
Millet GP; Institute of Sport Sciences, University of Lausanne, Lausanne, Switzerland.
Źródło:: Acta physiologica (Oxford, England) [Acta Physiol (Oxf)] 2021 Oct; Vol. 233 (2), pp. e13700. Date of Electronic Publication: 2021 Jun 19.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Oxford : Wiley-Blackwell
Original Publication: Oxford : Blackwell Pub., c2006-4
MeSH Terms:: Nitric Oxide*/metabolism
Nitric Oxide Synthase Type III*/metabolism
Animals ; Biological Availability ; Endothelium, Vascular/metabolism ; Hypoxia/metabolism ; Mice ; Mice, Inbred C57BL
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Contributed Indexing:: Keywords: endothelial function; exercise training; high-intensity; hypoxia; nitric oxide bioavailability
Substance Nomenclature:: 31C4KY9ESH (Nitric Oxide)
EC 1.14.13.39 (Nitric Oxide Synthase Type III)
Entry Date(s):: Date Created: 20210605 Date Completed: 20211028 Latest Revision: 20211028
Update Code:: 20240105
PubMed Central ID:: PMC8518730
DOI:: 10.1111/apha.13700
PMID:: 34089562
: Czasopismo naukowe

Pełny tekst

Aim: The optimal exercise intensity to improve endothelial function remains unclear, as well as whether the addition of hypoxia could potentiate this function. Therefore, the aim of this study was to compare the effects of different exercise intensities in normoxia and hypoxia on vascular reactivity and nitric oxide (NO) bioavailability in mice.
Methods: C57BL/6 mice underwent treadmill running three times per week, for 4 weeks at either low, maximal or supramaximal intensity in normoxia or hypoxia (inspire oxygen fraction = 0.13). Vascular reactivity and expression of genes and proteins involved in NO production/bioavailability were assessed in aorta using isolated vessel tension experiments, RT-qPCR and western blot, respectively. Circulating NO metabolites and pro-/antioxidant markers were measured.
Results: Hypoxic exercise improved both acetylcholine-induced vasorelaxation and phenylephrine-induced vasoconstriction compared to normoxic exercise, independently of intensity. In hypoxia, a higher acetylcholine-induced vasorelaxation was observed with high intensities (supramaximal and maximal) compared to low intensity. Exercise protocols modulated endothelial nitric oxide synthase (eNOS) and α1-adrenergic receptor (α 1 -AR) mRNA level, but not superoxide dismutase 3 (SOD3) and p47phox. No significant differences were observed for protein expression of α 1 -AR, total eNOS, phosphorylated eNOS, SOD isoforms and p47phox. However, plasma SOD and catalase activities were significantly increased in hypoxic supramaximal compared to hypoxic low intensity, while concentration of nitrotyrosine significantly decreased. The latter was also observed in hypoxic maximal and supramaximal compared to the same intensities in normoxia.
Conclusion: Hypoxic high-intensity exercise increases NO bioavailability and improves vascular function, opening promising clinical perspectives for cardiovascular disease prevention.
(© 2021 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society.)

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