-
Tytuł:
-
Proteomic profile of human stem cells from dental pulp and periodontal ligament.
-
Autorzy:
-
Lei T; Daxing Research Institute, University of Science and Technology Beijing, Beijing 100083, China; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Wang J; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Liu Y; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Chen P; Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Dongcheng District, Beijing 100700, China.
Zhang Z; Stomatology Department, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, China.
Zhang X; Daxing Research Institute, University of Science and Technology Beijing, Beijing 100083, China; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Guo W; Daxing Research Institute, University of Science and Technology Beijing, Beijing 100083, China; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Wang X; Stomatology Department, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191, China. Electronic address: .
Li Q; Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, China; Department of Immunology, Basic Medical College, Hebei Medical University, Shijiazhuang, Hebei 050017, China. Electronic address: .
Du H; Daxing Research Institute, University of Science and Technology Beijing, Beijing 100083, China; 112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China. Electronic address: .
-
Źródło:
-
Journal of proteomics [J Proteomics] 2021 Aug 15; Vol. 245, pp. 104280. Date of Electronic Publication: 2021 Jun 04.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Amsterdam : Elsevier
-
MeSH Terms:
-
Mesenchymal Stem Cells*
Periodontal Ligament*
Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Pulp ; Humans ; Proteomics ; Stem Cells
-
Contributed Indexing:
-
Keywords: 2DE; DPSCs; Nerve regeneration; PDLSCs; Quantitative proteomics
-
Entry Date(s):
-
Date Created: 20210605 Date Completed: 20210810 Latest Revision: 20210810
-
Update Code:
-
20240105
-
DOI:
-
10.1016/j.jprot.2021.104280
-
PMID:
-
34089896
-
Background The study of molecular profiling of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) contributes to understanding the high proliferation ability and multi-lineage differentiation potential. Objectives The aim of the study was to compare the protein abundance and specific markers of DPSCs and PDLSCs by protein profiles. Material and methods The DPSCs and PDLSCs extracted from the same tooth were lysed with 3 biological replicates and the protein was collected. Two-dimensional electrophoresis technology and TMT proteomics were used to separate and identify proteins. The data are available via ProteomeXchange with identifier PXD021997. The RT-qPCR detection of mRNA expression revealed a special marker for distinguishing two kinds of dental stem cells. Results Compared with PDLSCs, 962 differential proteins (DAPs) were up-regulated, and 127 were down-regulated in DPSCs. In the up-regulated DAPs, two high-scoring sub-networks were detected for neural-related molecules, which encode cell vesicle transport and mitochondrial energy transfer to regulate cell proliferation and secretion factors. A large number of cell adhesion molecules were distinguished among the highly expressed molecules of PDLSCs, supporting that stem cells provide cell attachment functions. It was interpreted ENPL, HS90A and HS90B were highly expressed in DPSCs, while CKB was highly abundant in PDLSCs. Another cell group confirmed that these molecules can be used as special biomarkers to identify and distinguish between DPSCs and PDLSCs. Conclusions This study can promote the basic research and clinical application of dental stem cells. Significance The high-throughput protein profiles were tested by combining two-dimensional gel proteomics and TMT-based proteomics. The proteomics of DPSCs and PDLSCs without individual difference demonstrated an accurate and comprehensive molecular expression profiles and interpretation of neural application potential, this study promotes the basic research of dental stem cells and clinical application.
(Copyright © 2021. Published by Elsevier B.V.)
