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Tytuł:
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Prostaglandin E2 promotes Staphylococcus aureus infection via EP4 receptor in bovine endometrium.
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Autorzy:
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Liu K; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China; Laboratory of Veterinary Pathology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: .
Mao W; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: .
Liu B; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: .
Li T; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: .
Wang X; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: .
Pei L; Veterinary Research Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot, China. Electronic address: .
Cao J; Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: .
Wang F; Laboratory of Veterinary Pathology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: .
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Źródło:
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Microbial pathogenesis [Microb Pathog] 2021 Sep; Vol. 158, pp. 105019. Date of Electronic Publication: 2021 Jun 06.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; Orlando : Academic Press, c1986-
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MeSH Terms:
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Dinoprostone*
Staphylococcal Infections*/veterinary
Animals ; Cattle ; Endometrium ; Female ; Receptors, Prostaglandin E, EP2 Subtype ; Staphylococcus aureus
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Contributed Indexing:
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Keywords: Bovine endometritis; EP4 receptor; Infection; Prostaglandin E2; S. aureus
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Substance Nomenclature:
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0 (Receptors, Prostaglandin E, EP2 Subtype)
K7Q1JQR04M (Dinoprostone)
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Entry Date(s):
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Date Created: 20210609 Date Completed: 20210818 Latest Revision: 20210818
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Update Code:
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20240104
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DOI:
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10.1016/j.micpath.2021.105019
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PMID:
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34107344
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Prostaglandin E2 (PGE 2 ) enhances Staphylococcus aureus infection but its mechanism is not well understood. Here, we examined the effect of PGE 2 on Staphylococcal Protein A (SPA) expression in bovine endometrium and determined the role of select PGE 2 receptors (i.e., EP2 and EP4) in adhesion and internalization of S. aureus. S. aureus isolate SA113 was used for in vitro infection of bovine endometrial tissues and epithelial cells, with treatment conditions consisting of untreated control, SA113 treatment, SA113 + PGE 2 , SA113 + PGE 2 + EP2 receptor antagonist (AH-6809), and SA113 + PGE 2 + EP4 receptor antagonist (AH-23848). Immunofluorescence assay revealed that PGE 2 could promote SPA expression in S. aureus-infected bovine endometrial tissues. PGE 2 also enhanced the adhesion and internalization of S. aureus in bovine endometrial cells. The addition of EP4 antagonist, but not the EP2 antagonist, abrogated the ability of PGE 2 to promote S. aureus SPA expression, adhesion, and internalization in endometrial cells. Our findings suggest that S. aureus infection in the endometrium is enhanced by PGE 2 through the EP4 receptor. This result is essential for the development of new approach to treating S. aureus infection, such as the application of EP4 antagonist as an adjunct drug treatment.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
