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Tytuł pozycji:

Clinical and in Vitro Evidence against Placenta Infection at Term by Severe Acute Respiratory Syndrome Coronavirus 2.

Tytuł:
Clinical and in Vitro Evidence against Placenta Infection at Term by Severe Acute Respiratory Syndrome Coronavirus 2.
Autorzy:
Colson A; Pole of Obstetrics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium; Pole of Pharmacology and Therapeutics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium; Division of Obstetrics, Saint-Luc University Hospital, Brussels, Belgium. Electronic address: .
Depoix CL; Pole of Obstetrics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Dessilly G; Medical Microbiology, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Baldin P; Department of Pathology, Saint-Luc University Hospital, Brussels, Belgium.
Danhaive O; Division of Neonatology, Saint-Luc University Hospital, Brussels, Belgium; Department of Pediatrics, Benioff Children's Hospital, University of California, San Francisco, San Francisco, California.
Hubinont C; Pole of Obstetrics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium; Division of Obstetrics, Saint-Luc University Hospital, Brussels, Belgium.
Sonveaux P; Pole of Pharmacology and Therapeutics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Debiève F; Pole of Obstetrics, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium; Division of Obstetrics, Saint-Luc University Hospital, Brussels, Belgium.
Źródło:
The American journal of pathology [Am J Pathol] 2021 Sep; Vol. 191 (9), pp. 1610-1623. Date of Electronic Publication: 2021 Jun 08.
Typ publikacji:
Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: 2011-: New York : Elsevier
Original Publication: Philadelphia [etc.] American Assn. of Pathologists [etc.]
MeSH Terms:
COVID-19*/enzymology
COVID-19*/pathology
Gene Expression Regulation, Enzymologic*
Placenta Diseases*/enzymology
Placenta Diseases*/pathology
Pregnancy Complications, Infectious*/enzymology
Pregnancy Complications, Infectious*/pathology
Trophoblasts*/enzymology
Trophoblasts*/pathology
Virus Internalization*
Angiotensin-Converting Enzyme 2/*biosynthesis
SARS-CoV-2/*metabolism
Serine Endopeptidases/*biosynthesis
Adult ; Female ; Humans ; Pregnancy
Substance Nomenclature:
EC 3.4.17.23 (ACE2 protein, human)
EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
EC 3.4.21.- (Serine Endopeptidases)
EC 3.4.21.- (TMPRSS2 protein, human)
Entry Date(s):
Date Created: 20210610 Date Completed: 20210830 Latest Revision: 20210830
Update Code:
20240104
PubMed Central ID:
PMC8184362
DOI:
10.1016/j.ajpath.2021.05.009
PMID:
34111431
Czasopismo naukowe
Despite occasional reports of vertical transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during pregnancy, the question of placental infection and its consequences for the newborn remain unanswered. Herein, we analyzed the placentas of 31 coronavirus disease 2019-positive mothers by reverse transcriptase PCR, immunohistochemistry, and in situ hybridization. Only one case of placental infection was detected, which was associated with intrauterine demise of the fetus. Differentiated primary trophoblasts were then isolated from nonpathologic human placentas at term, differentiated, and exposed to SARS-CoV-2 virions. Unlike for positive control cells Vero E6, the virus inside cytotrophoblasts and syncytiotrophoblasts or in the supernatant 4 days after infection was undetectable. As a mechanism of defense, we hypothesized that trophoblasts at term do not express angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), the two main host membrane receptors for SARS-CoV-2 entry. The quantification of these proteins in the placenta during pregnancy confirmed the absence of TMPRSS2 at the surface of the syncytium. Surprisingly, a transiently induced experimental expression of TMPRSS2 did not allow the entry or replication of the virus in differentiated trophoblasts. Altogether, these results underline that trophoblasts are not likely to be infected by SARS-CoV-2 at term, but raise concern about preterm infection.
(Copyright © 2021 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)

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