Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Characterization of APX and APX-R gene family in Brassica juncea and B. rapa for tolerance against abiotic stresses.

Tytuł:
Characterization of APX and APX-R gene family in Brassica juncea and B. rapa for tolerance against abiotic stresses.
Autorzy:
Verma D; Department of Biotechnology, BMS Block I, Panjab University, Sector 25, Chandigarh, 160014, India.
Upadhyay SK; Department of Botany, Panjab University, Sector 14, Chandigarh, 160014, India.
Singh K; Department of Biotechnology, BMS Block I, Panjab University, Sector 25, Chandigarh, 160014, India. .
Źródło:
Plant cell reports [Plant Cell Rep] 2022 Mar; Vol. 41 (3), pp. 571-592. Date of Electronic Publication: 2021 Jun 11.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Berlin ; New York : Springer, 1981-
MeSH Terms:
Gene Expression Regulation, Plant*/genetics
Mustard Plant*/genetics
Ascorbate Peroxidases/genetics ; Ascorbate Peroxidases/metabolism ; Gene Expression Profiling ; Genes, vpr ; Hydrogen Peroxide/metabolism ; Multigene Family ; Phylogeny ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Stress, Physiological/genetics
References:
Akbudak MA, Filiz E, Vatansever R, Kontbay K (2018) Genome-wide identification and expression profiling of ascorbate peroxidase (APX) and glutathione peroxidase (GPX) genes under drought stress in sorghum (Sorghum bicolor L.). J Plant Growth Regul 37:925–936.
Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107:1049. (PMID: 12228418157235)
Armenteros JJA, Tsirigos KD, Sønderby CK, Petersen TN, Winther O, Brunak S, von Heijne G, Nielsen H (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks. Nat Biotechnol 37:420–423.
Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, Ren J, Li WW, Noble WS (2009) MEME {SUITE}: tools for motif discovery and searching. Nucleic Acids Res 37:W202–W208. https://doi.org/10.1093/nar/gkp335. (PMID: 10.1093/nar/gkp335194581582703892)
Bhardwaj AR, Joshi G, Kukreja B, Malik V, Arora P, Pandey R, Shukla RN, Bankar KG, Katiyar-Agarwal S, Goel S (2015) Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea. BMC Plant Biol 15:9. (PMID: 256046934310166)
Bhatt D, Saxena SC, Jain S, Dobriyal AK, Majee M, Arora S (2013) Cloning, expression and functional validation of drought inducible ascorbate peroxidase (Ec-apx1) from Eleusine coracana. Mol Biol Rep 40:1155–1165. (PMID: 23065288)
Caverzan A, Passaia G, Rosa SB, Ribeiro CW, Lazzarotto F, Margis-Pinheiro M (2012) Plant responses to stresses: role of ascorbate peroxidase in the antioxidant protection. Genet Mol Biol 35:1011–1019. (PMID: 234127473571416)
Chandna R, Augustine R, Bisht NC (2012) Evaluation of candidate reference genes for gene expression normalization in Brassica juncea using real time quantitative RT-PCR. PLoS ONE 7:e36918. (PMID: 226063083350508)
Chen C, Letnik I, Hacham Y, Dobrev P, Ben-Daniel BH, Vanková R, Amir R, Miller G (2014) ASCORBATE PEROXIDASE6 protects Arabidopsis desiccating and germinating seeds from stress and mediates cross talk between reactive oxygen species, abscisic acid, and auxin. Plant Physiol 166:370–383. (PMID: 250493614149721)
Chen M, Li K, Li H, Song CP, Miao Y (2017) The glutathione peroxidase gene family in gossypium hirsutum: genome-wide identification, classification, gene expression and functional analysis. Sci Rep 7:44743. (PMID: 283001955353742)
Cheng F, Liu S, Wu J, Fang L, Sun S, Liu B, Li P, Hua W, Wang X (2011) BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biol 11:1–6.
Chin DC, Kumar RS, Suen CS, Chien CY, Hwang MJ, Hsu CH, Xuhan X, Lai ZX, Yeh KW (2019) Plant cytosolic ascorbate peroxidase with dual catalytic activity modulates abiotic stress tolerances. Iscience 16:31–49. (PMID: 311461306542772)
Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676. (PMID: 16081474)
Das K, Roychoudhury A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci 2:53.
Davletova S, Rizhsky L, Liang H, Shengqiang Z, Oliver DJ, Coutu J, Shulaev V, Schlauch K, Mittler R (2005) Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17:268–281. (PMID: 15608336544504)
Dunand C, Mathé C, Lazzarotto F, Margis R, Margis-Pinheiro M (2011) Ascorbate peroxidase-related (APx-R) is not a duplicable gene. Plant Signal Behav 6:1908–1913. (PMID: 222312003337176)
Emanuelsson O, Nielsen H, Von HG (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci 8:978–984. (PMID: 103380082144330)
Fi̇li̇z E, Tombuloğlu H (2015) Genome-wide distribution of superoxide dismutase (SOD) gene families in Sorghum bicolor. Turk J Biol 39:49–59.
Gao Y, Liu J, Yang F, Zhang G, Wang D, Zhang L, Ou Y, Yao Y (2020) The WRKY transcription factor WRKY8 promotes resistance to pathogen infection and mediates drought and salt stress tolerance in Solanum lycopersicum. Physiol Plant 168:98–117. (PMID: 31017672)
Gasteiger E, Hoogland C, Gattiker A, Wilkins MR, Appel RD, Bairoch A (2005) Protein identification and analysis tools on the ExPASy server. In: Walker JM (ed) The proteomics protocols handbook. Springer Protocols Handbooks, Humana Press, pp 571–607. https://doi.org/10.1385/1-59259-890-0.
Ghawana S, Paul A, Kumar H, Kumar A, Singh H, Bhardwaj PK, Rani A, Singh RS, Raizada J, Singh K (2011) An RNA isolation system for plant tissues rich in secondary metabolites. BMC Res Notes 4:85. (PMID: 214437673079660)
Gómez-Campo C (1980) Morphology and morpho-taxonomy of the tribe Brassiceae. In: Tsunoda S, Hinata K, Gómez-Campo C (eds) Brassica crops and wild allies, biology and breeding. Japan Scientific Societies Press, Tokyo, pp 3–31.
Guo YM, Samans B, Chen S, Kibret KB, Hatzig S, Turner NC, Nelson MN, Cowling WA, Snowdon RJ (2017) Drought-tolerant Brassica rapa shows rapid expression of gene networks for general stress responses and programmed cell death under simulated drought stress. Plant Mol Biol Report 35:416–430. (PMID: 287518015504209)
Gupta S, Dong Y, Dijkwel PP, Mueller-Roeber B, Gechev TS (2019) Genome-wide analysis of ROS antioxidant genes in resurrection species suggest an involvement of distinct ROS detoxification systems during desiccation. Int J Mol Sci 20:3101. (PMID: 6627786)
Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, Couger MB, Eccles D, Li B, Lieber M (2013) De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc 8:1494. (PMID: 23845962)
Hayat S, Masood A, Yusuf M, Fariduddin Q, Ahmad A (2009) Growth of Indian mustard (Brassica juncea L.) in response to salicylic acid under high-temperature stress. Brazil J Plant Physiol 21:187–195.
Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res 27:297–300. (PMID: 9847208148163)
Hong SH, Tripathi BN, Chung MS, Cho C, Lee S, Kim JH, Bai HW, Bae HJ, Cho JY, Chung BY (2018) Functional switching of ascorbate peroxidase 2 of rice (OsAPX2) between peroxidase and molecular chaperone. Sci Rep 8:1–9.
Hu B, Jin J, Guo AY, Zhang H, Luo J, Gao G (2015) GSDS 2.0: an upgraded gene feature visualization server. Bioinformatics 31:1296–1297.
Huang D, Wu W, Abrams SR, Cutler AJ (2008) The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J Exp Bot 59:2991–3007. (PMID: 185523552504347)
Ishikawa T, Takeda T, Shigeoka S (1996) Purification and characterization of cytosolic ascorbate peroxidase from komatsuna (Brassica rapa). Plant Sci 120:11–18.
Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C, McWilliam H, Maslen J, Mitchell A, Nuka G (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30:1236–1240. (PMID: 244516263998142)
Kapustin Y, Souvorov A, Tatusova T, Lipman D (2008) Splign: algorithms for computing spliced alignments with identification of paralogs. Biol Dir 3:20.
Kim MD, Kim Y, Kwon S, Yun D, Kwak S, Lee H (2010) Enhanced tolerance to methyl viologen-induced oxidative stress and high temperature in transgenic potato plants overexpressing the CuZnSOD, APX and NDPK2 genes. Physiol Plant 140:153–162. (PMID: 20553417)
Korkuć P, Schippers JHM, Walther D (2014) Characterization and identification of cis-regulatory elements in Arabidopsis based on single-nucleotide polymorphism information. Plant Physiol 164:181–200. (PMID: 24204023)
Koscielny CB, Hazebroek J, Duncan RW (2018) Phenotypic and metabolic variation among spring Brassica napus genotypes during heat stress. Crop Pasture Sci 69:284–295.
Koussevitzky S, Suzuki N, Huntington S, Armijo L, Sha W, Cortes D, Shulaev V, Mittler R (2008) Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J Biol Chem 283:34197–34203. (PMID: 188522642590703)
Kubo A, Saji H, Tanaka K, Tanaka K, Kondo N (1992) Cloning and sequencing of a cDNA encoding ascorbate peroxidase from Arabidopsis thaliana. Plant Mol Biol 18:691–701. (PMID: 1558944)
Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306. (PMID: 18417537)
Kurilich AC, Tsau GJ, Brown A, Howard L, Klein BP, Jeffery EH, Kushad M, Wallig MA, Juvik JA (1999) Carotene, tocopherol, and ascorbate contents in subspecies of Brassica oleracea. J Agric Food Chem 47:1576–1581. (PMID: 10564019)
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685. (PMID: 5432063)
Lazzarotto F, Teixeira FK, Rosa SB, Dunand C, Fernandes CL, de Vasconcelos FA, Silveira JAG, Verli H, Margis R, Margis-Pinheiro M (2011) Ascorbate peroxidase-related (APx-R) is a new heme-containing protein functionally associated with ascorbate peroxidase but evolutionarily divergent. New Phytol 191:234–250. (PMID: 21352234)
Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325–327. (PMID: 1175232711752327)
Letunic I, Doerks T, Bork P (2015) SMART: recent updates, new developments and status in 2015. Nucleic Acids Res 43:D257–D260. (PMID: 25300481)
Liao GL, Liu Q, Li YQ, Zhong M, Huang CH, Jia DF, Xu XB (2020) Identification and expression profiling analysis of ascorbate peroxidase gene family in Actinidia chinensis (Hongyang). J Plant Res 133:715–726. (PMID: 32506283)
Liu JX, Feng K, Duan AQ, Li H, Yang QQ, Xu ZS, Xiong AS (2019) Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis. BMC Plant Biol 19:1–13.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25:402–408. (PMID: 11846609)
Lu Z, Liu D, Liu S (2007) Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis. Plant Cell Rep 26:1909–1917. (PMID: 17571267)
Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR (2010) CDD: a conserved domain database for the functional annotation of proteins. Nucleic Acids Res 39:D225–D229. (PMID: 211095323013737)
Mishra S, Das BA (2004) Effect of short-term exposure to NaCl on photochemical activity and antioxidant enzymes in Bruguiera parviflora, a non-secretor mangrove. Acta Physiol Plant 26:317.
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410. (PMID: 12234732)
Mittler R, Zilinskas BA (1993) Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Anal Biochem 212:540–546. (PMID: 8214598)
Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498. (PMID: 15465684)
Mohammadian MA, Largani ZK, Sajedi RH (2012) Quantitative and qualitative comparison of antioxidant activity in the flavedo tissue of three cultivars of citrus fruit under cold stress. Aust J Crop Sci 6:402.
Mullen RT, Trelease RN (2000) The sorting signals for peroxisomal membrane-bound ascorbate peroxidase are within its C-terminal tail. J Biol Chem 275:16337–16344. (PMID: 10748009)
Nagaharu U (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452.
Najami N, Janda T, Barriah W, Kayam G, Tal M, Guy M, Volokita M (2008) Ascorbate peroxidase gene family in tomato: its identification and characterization. Mol Genet Genom 279:171–182.
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880.
Østergaard L, King GJ (2008) Standardized gene nomenclature for the Brassica genus. Plant Methods 4:1–4.
Ozyigit II, Filiz E, Vatansever R, Kurtoglu KY, Koc I, Öztürk MX, Anjum NA (2016) Identification and comparative analysis of H2O2-scavenging enzymes (ascorbate peroxidase and glutathione peroxidase) in selected plants employing bioinformatics approaches. Front Plant Sci 7:301. (PMID: 270474984802093)
Padaria JC, Vishwakarma H, Biswas K, Jasrotia RS, Singh GP (2014) Molecular cloning and in-silico characterization of high temperature stress responsive pAPX gene isolated from heat tolerant Indian wheat cv. Raj 3765. BMC Res Notes 7:1–12.
Panchuk II, Zentgraf U, Volkov RA (2005) Expression of the Apx gene family during leaf senescence of Arabidopsis thaliana. Planta 222:926–932. (PMID: 16034597)
Panchy N, Lehti-Shiu M, Shiu SH (2016) Evolution of gene duplication in plants. Plant Physiol 171:2294–2316. (PMID: 272883664972278)
Park SY, Ryu SH, Jang IC, Kwon SY, Kim JG, Kwak SS (2004) Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweetpotato and its expression in response to stress. Mol Genet Genomics 271:339–346. (PMID: 14986108)
Patterson WR, Poulos TL (1995) Crystal structure of recombinant pea cytosolic ascorbate peroxidase. Biochemistry 34:4331–4341. (PMID: 7703247)
Pavlović I, Mlinarić S, Tarkowská D, Oklestkova J, Novák O, Lepeduš H, Bok VV, Brkanac SR, Strnad M, Salopek-Sondi B (2019) Early Brassica crops responses to salinity stress: a comparative analysis between Chinese cabbage, white cabbage, and kale. Front Plant Sci 10:450. (PMID: 310317866470637)
Satoh R, Nakashima K, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2002) ACTCAT, a novel cis-acting element for proline-and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis. Plant Physiol 130:709–719. (PMID: 12376638166600)
Shafi A, Dogra V, Gill T, Ahuja PS, Sreenivasulu Y (2014) Simultaneous over-expression of PaSOD and RaAPX in transgenic Arabidopsis thaliana confers cold stress tolerance through increase in vascular lignifications. PLoS ONE 9:e1103002.
Sharp KH, Mewies M, Moody PCE, Raven EL (2003) Crystal structure of the ascorbate peroxidase–ascorbate complex. Nat Struct Mol Biol 10:303–307.
Shi WM, Muramoto Y, Ueda A, Takabe T (2001) Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana. Gene 273:23–27. (PMID: 11483357)
Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53:1305–1319. (PMID: 11997377)
Sigrist CJA, De Castro E, Cerutti L, Cuche BA, Hulo N, Bridge A, Bougueleret L, Xenarios I (2012) New and continuing developments at PROSITE. Nucleic Acids Res 41:D344–D347. (PMID: 231616763531220)
Sonnhammer ELL, Von Heijne G, Krogh A (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. ISMB proc Bioinform J 6:175–182.
Tao C, Jin X, Zhu L, Xie Q, Wang X, Li H (2018) Genome-wide investigation and expression profiling of APX gene family in Gossypium hirsutum provide new insights in redox homeostasis maintenance during different fiber development stages. Mol Genet Genom 293:685–697.
Teixeira FK, Menezes-Benavente L, Margis R, Margis-Pinheiro M (2004) Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome. J Mol Evol 59:761–770. (PMID: 15599508)
Tsunoda S (1980) Eco-physiology of wild and cultivated forms in Brassica and allied genera. In: Tsunoda S, Hinata K, Gomez-Campo C (eds) Brassica crops and wild allies, biology and breeding. Japan Scientific Societies Press, Tokyo, pp 109–120.
Tyagi S, Verma PC, Singh K, Upadhyay SK (2020) Molecular characterization of ascorbate peroxidase (APX) and APX-related (APX-R) genes in Triticum aestivum L. Genomics 112:4208–4223. (PMID: 32681868)
Verma D, Lakhanpal N, Singh K (2019) Genome-wide identification and characterization of abiotic-stress responsive SOD (superoxide dismutase) gene family in Brassica juncea and B. rapa. BMC Genom 20:1–18.
Wada K, Tada T, Nakamura Y, Ishikawa T, Yabuta Y, Yoshimura K, Shigeoka S, Nishimura K (2003) Crystal structure of chloroplastic ascorbate peroxidase from tobacco plants and structural insights into its instability. J Biochem 134:239–244. (PMID: 12966073)
Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun JH, Bancroft I, Cheng F (2011) The genome of the mesopolyploid crop species Brassica rapa. Nat Genet 43:1035. (PMID: 21873998)
Wang S, Zhou G, Huang X, Hu J, Wang B, Lin C, Li X, Jia Y, Wang A (2017) Transcriptome analysis of non-heading Chinese cabbage under heat stress by RNA-seq and marker identification. Euphytica 213:109.
Wei Z, Wang M, Chang S, Wu C, Liu P, Meng J, Zou J (2016) Introgressing subgenome components from Brassica rapa and B. carinata to B. juncea for broadening its genetic base and exploring intersubgenomic heterosis. Front Plant Sci 7:1677. (PMID: 279094405112257)
Wu B, Wang B (2019) Comparative analysis of ascorbate peroxidases (APXs) from selected plants with a special focus on Oryza sativa employing public databases. PLoS ONE 14:e0226543. (PMID: 318562326922425)
Xiu Y, Yanchao XU, Fangfang Y, Magwanga RO, Xiaoyan CAI, Xingxing W, Yuhong W, Yuqing HOU, Kunbo W, Fang LIU (2019) Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum. J Cott Res 2:11.
Xu Z (2017) Cruciferae. In: Xu Z, Deng M (eds) Identification and control of common weeds, vol 2. Springer, pp 437–474.
Yang J, Yan R, Roy A, Xu D, Poisson J, Zhang Y (2015) The I-TASSER Suite: protein structure and function prediction. Nat Methods 12:7–8. (PMID: 255492654428668)
Yoshimura K, Yabuta Y, Ishikawa T, Shigeoka S (2000) Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiol 123:223–234. (PMID: 1080623958996)
Young LW, Wilen RW, Bonham-Smith PC (2004) High temperature stress of Brassica napus during flowering reduces micro-and megagametophyte fertility, induces fruit abortion, and disrupts seed production. J Exp Bot 55:485–495. (PMID: 14739270)
Yu C, Lin C, Hwang J (2004) Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions. Protein Sci 13:1402–1406. (PMID: 150966402286765)
Zhang Z, Zhang Q, Wu J, Zheng X, Zheng S, Sun X, Qiu Q, Lu T (2013) Gene knockout study reveals that cytosolic ascorbate peroxidase 2 (OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses. PLoS ONE 8:e574472.
Zhou Y, Hu L, Ye S, Jiang L, Liu S (2018) Genome-wide identification of glutathione peroxidase (GPX) gene family and their response to abiotic stress in cucumber. 3 Biotech 8:159. (PMID: 295159655834408)
Contributed Indexing:
Keywords: APX activity; Ascorbate peroxidase; Brassica; Cloning and characterization; Gene expression; Overexpression
Substance Nomenclature:
0 (Plant Proteins)
BBX060AN9V (Hydrogen Peroxide)
EC 1.11.1.11 (Ascorbate Peroxidases)
Entry Date(s):
Date Created: 20210611 Date Completed: 20220411 Latest Revision: 20220411
Update Code:
20240104
DOI:
10.1007/s00299-021-02726-0
PMID:
34115169
Czasopismo naukowe
Key Message: APX and APX-R gene families were identified and characterized in two important oilseed species of Brassica. Gene expression under abiotic stress conditions, recombinant protein expression, and analysis further divulged their drought, heat, and salt-responsive behavior. Ascorbate peroxidases (APX) are heme-dependent enzymes that rid the cells of H 2 O 2 and regulate diverse biological processes. In the present study, we performed APX gene family characterization in two Brassica sp. (B. juncea and B. rapa) as these are commercially important oilseed crops and affected severely by abiotic stresses. We identified 16 BjuAPX and 9 BraAPX genes and 2 APX-R genes each in B. juncea and B. rapa genomes, respectively. Phylogenetic analysis divided the APX genes into five distinct clades, which exhibited conservation in the gene structure, motif organization, and sub-cellular location within the clade. Structural analysis of APX and APX-R proteins revealed the amino acid substitutions in conserved domains of APX-R proteins. The expression profiling of BjuAPX and BraAPX genes showed that 3 BjuAPX, 7BraAPX, and 2 BraAPX-R genes were drought and heat responsive. Notably, BjuAAPX1a, BjuAPX1d, BjuAAPX6, BraAAPX1a, BraAAPX2, and BraAAPX3b showed high expression levels in RT-qPCR. Cis-regulatory elements in APX and APX-R gene promoters supported the differential behavior of these genes. Further, two stress-responsive genes BjuAPX1d and BraAAPX2 were cloned, characterized, and their roles were validated under heat, drought, salt, and cold stress in bacterial expression system. This study for the first time reports the presence of APX activity in dimeric and LMW form of purified BraAAPX2 protein. The study may help pave way for developing abiotic stress-tolerant Brassica crops.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies