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Tytuł:
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Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana.
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Autorzy:
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Feng Y; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
Cui R; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
Huang Y; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
Shi L; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
Wang S; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
Xu F; National Key Laboratory of Crop Genetic Improvement, and Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: .
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Źródło:
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Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2021 Sep 01; Vol. 220, pp. 112406. Date of Electronic Publication: 2021 Jun 10.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam, Netherlands : Elsevier
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MeSH Terms:
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Arabidopsis*/drug effects
Arabidopsis*/genetics
Arabidopsis*/growth & development
Arabidopsis*/metabolism
Arabidopsis Proteins*/genetics
Arabidopsis Proteins*/metabolism
Boron*/metabolism
Boron*/toxicity
Transcription Factors*/genetics
Transcription Factors*/metabolism
Gene Expression Regulation, Plant ; Mutation ; Phenotype ; Plants, Genetically Modified/drug effects ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Adaptation, Physiological/genetics
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Contributed Indexing:
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Keywords: Arabidopsis; Boron toxicity; High B tolerance; Transcription factor; WRKY47
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Substance Nomenclature:
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0 (Arabidopsis Proteins)
N9E3X5056Q (Boron)
0 (Transcription Factors)
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Entry Date(s):
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Date Created: 20210613 Date Completed: 20240209 Latest Revision: 20240209
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Update Code:
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20240209
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DOI:
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10.1016/j.ecoenv.2021.112406
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PMID:
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34119927
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Boron (B) excess gives rise to a serious agricultural problem. In this study, we identified a B toxicity responsive transcription factor AtWRKY47 in Arabidopsis thaliana. The T-DNA insertion mutants Atwrky47 showed enhanced tolerance to B toxicity with better growth parameters under high B conditions compared to wild-type Col-0 plants. Quantitative analysis of AtWRKY47 mRNA abundance indicated that it was down-regulated under B toxicity conditions. Fluorescently labeled AtWRKY47 protein was localized in nucleus. In contrast to the phenotype of Atwrky47 mutants, overexpression of AtWRKY47 in Col-0 background resulted in lower biomass, less chlorophyll content, and increased sensitivity to B toxicity. More importantly, the B concentration in shoots was higher in the overexpression lines and lower in the Atwrky47 mutants than in Col-0 plants, respectively. These results demonstrate that AtWRKY47 gene plays a key role in regulating plant tolerance to B toxicity.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
