Circ-SPG11 knockdown hampers IL-1β-induced osteoarthritis progression via targeting miR-337-3p/ADAMTS5.

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Abstrakt

Tytuł:: Circ-SPG11 knockdown hampers IL-1β-induced osteoarthritis progression via targeting miR-337-3p/ADAMTS5.
Autorzy:: Liu Y; Department of Orthopedics, Shijiazhuang People's Hospital, No. 365 Jianhua South Road, Shijiazhang, Hebei, 050000, People's Republic of China.
Li Q; Department of Orthopedics, Shijiazhuang People's Hospital, No. 365 Jianhua South Road, Shijiazhang, Hebei, 050000, People's Republic of China.
Gao Z; Department of Orthopedics, Shijiazhuang People's Hospital, No. 365 Jianhua South Road, Shijiazhang, Hebei, 050000, People's Republic of China.
Lei F; Department of Orthopedics, Shijiazhuang People's Hospital, No. 365 Jianhua South Road, Shijiazhang, Hebei, 050000, People's Republic of China. .
Gao X; Department of Orthopedics, Shijiazhuang People's Hospital, No. 365 Jianhua South Road, Shijiazhang, Hebei, 050000, People's Republic of China.
Źródło:: Journal of orthopaedic surgery and research [J Orthop Surg Res] 2021 Jun 17; Vol. 16 (1), pp. 392. Date of Electronic Publication: 2021 Jun 17.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, 2006-
MeSH Terms:: Gene Knockdown Techniques*
ADAMTS5 Protein/*genetics
ADAMTS5 Protein/*metabolism
Interleukin-1beta/*adverse effects
MicroRNAs/*genetics
MicroRNAs/*metabolism
Osteoarthritis, Knee/*genetics
Proteins/*genetics
RNA, Circular/*genetics
Cell Line ; Disease Progression ; Down-Regulation/genetics ; Gene Expression Regulation/genetics ; Humans ; Osteoarthritis, Knee/etiology ; Proteins/physiology ; RNA, Circular/physiology ; Up-Regulation/genetics
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Contributed Indexing:: Keywords: ADAMTS5; Circ-SPG11; IL-1β; MiR-337-3p; Osteoarthritis
Substance Nomenclature:: 0 (Interleukin-1beta)
0 (MicroRNAs)
0 (Mirn337 microRNA, human)
0 (Proteins)
0 (RNA, Circular)
0 (SPG11 protein, human)
EC 3.4.24.- (ADAMTS5 Protein)
EC 3.4.24.- (ADAMTS5 protein, human)
Entry Date(s):: Date Created: 20210618 Date Completed: 20211110 Latest Revision: 20211110
Update Code:: 20240104
PubMed Central ID:: PMC8212518
DOI:: 10.1186/s13018-021-02526-y
PMID:: 34140036
: Czasopismo naukowe

Pełny tekst

Background: Osteoarthritis (OA) is responsible for the impotent disability in old people. Circular RNA (circRNA) has been reported to be related to the development of diseases. The lack of research on the role of circRNA spastic paraplegia 11 (circ-SPG11) results in conducting this study.
Methods: The expression of circ-SPG11, microRNA-337-3p (miR-337-3p), and aggrecanases like a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) mRNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was used to measure the protein expression of extracellular matrix (ECM) degradation-related markers and ADAMTS5. Ribonuclease R (RNase R) was applied to test the stability of circ-SPG11 in CHON-001 cells. The viability, apoptosis, TNF-α and IL-6 production were determined by cell counting kit-8 (CCK-8) assay, flow cytometry assay, and enzyme-linked immunosorbent assay (ELISA), respectively. Meanwhile, the interaction between miR-337-3p and circ-SPG11 or ADAMTS5 was respectively predicted by Circinteractome or Starbase2.0, which was further verified by dual-luciferase reporter system and RNA binding protein immunoprecipitation (RIP) assay.
Results: Circ-SPG11 and ADAMTS5 were upregulated and miR-337-3p was downregulated in OA tissues and OA model cells. Circ-SPG11 knockdown allayed interleukin 1β (IL-1β)-induced restraint in viability and promotion in apoptosis, TNF-α, and IL-6 generation and ECM degradation in CHON-001 cells. Anti-miR-337-3p or ADAMTS5 overexpression correspondingly reversed si-circ-SPG11 or miR-337-3p overexpression-mediated facilitation in viability, and inhibition in apoptosis, TNF-α and IL-6 generation and ECM degradation in OA model cells. Moreover, anti-miR-337-3p ameliorated si-circ-SPG11-mediated inhibition in ADAMTS5 mRNA and protein expression in OA model cells.
Conclusion: Circ-SPG11 facilitated OA development via regulating miR-337-3p/ADAMTS5 axis. This finding might contribute to the improvement of OA therapy.

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