The Application of the Tissue Microarray (TMA) Technology to Analyze Cerebral Organoids.

Szczegóły
Abstrakt

Tytuł:: The Application of the Tissue Microarray (TMA) Technology to Analyze Cerebral Organoids.
Autorzy:: Biunno I; Integrated Systems Engineering, Milano, Italy.; Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Milano, Italy.
Paiola E; Division of Anatomy Pathology, IRCCS MultiMedica, Milano, Italy.
De Blasio P; Integrated Systems Engineering, Milano, Italy.
Źródło:: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society [J Histochem Cytochem] 2021 Jul; Vol. 69 (7), pp. 451-460. Date of Electronic Publication: 2021 Jun 18.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: <2011->: Thousand Oaks, CA : SAGE Publications
Original Publication: Baltimore : Williams & Wilkins Co., c1953-
MeSH Terms:: Tissue Array Analysis*
Brain/*cytology
Organoids/*cytology
Cell Differentiation ; Humans ; Induced Pluripotent Stem Cells/cytology ; Neurons/cytology
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Contributed Indexing:: Keywords: biomarker analysis; human-induced pluripotent stem cells (hiPSC); neuronal differentiation; organoids; tissue microarrays
Entry Date(s):: Date Created: 20210618 Date Completed: 20211006 Latest Revision: 20240402
Update Code:: 20240402
PubMed Central ID:: PMC8246530
DOI:: 10.1369/00221554211025327
PMID:: 34142588
: Czasopismo naukowe

"Multi-Omics" technologies have contributed greatly to the understanding of various diseases by enabling researchers to accurately and rapidly investigate the molecular circuitry that connects cellular systems. The tissue-engineered, three-dimensional (3D), in vitro disease model "organoid" integrates the "omics" results in a model system, elucidating the complex links between genotype and phenotype. These 3D structures have been used to model cancer, infectious disease, toxicity, and neurological disorders. Here, we describe the advantage of using the tissue microarray (TMA) technology to analyze human-induced pluripotent stem cell-derived cerebral organoids. Compared with the conventional processing of individual samples, sectioning and staining of TMA slides are faster and can be automated, decreasing labor and reagent costs. The TMA technology faithfully captures cell morphology variations and detects specific biomarkers. The use of this technology can scale up organoid research results in at least two ways: (1) in the number of specimens that can be analyzed simultaneously and (2) in the number of consecutive sections that can be produced for analysis with different probes and antibodies.

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