-
Tytuł:
-
On-line screening of indoleamine 2,3-dioxygenase 1 inhibitors by partial filling capillary electrophoresis combined with rapid polarity switching.
-
Autorzy:
-
Liu Z; State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Wang C; State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Wang X; State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: .
Van Schepdael A; Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven - University of Leuven, Leuven, Belgium. Electronic address: .
-
Źródło:
-
Journal of chromatography. A [J Chromatogr A] 2021 Aug 16; Vol. 1651, pp. 462305. Date of Electronic Publication: 2021 Jun 18.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Amsterdam ; New York : Elsevier, 1993-
-
MeSH Terms:
-
Electrophoresis, Capillary*
Chemistry Techniques, Analytical/*methods
Drug Evaluation, Preclinical/*methods
Enzyme Inhibitors/*isolation & purification
Indoleamine-Pyrrole 2,3,-Dioxygenase/*antagonists & inhibitors
Biological Products/chemistry ; Enzyme Activation/drug effects ; Enzyme Inhibitors/pharmacology ; Humans
-
Contributed Indexing:
-
Keywords: Capillary electrophoresis; Indoleamine 2,3-dioxygenase 1 Inhibitors; Natural products; On-line screening
-
Substance Nomenclature:
-
0 (Biological Products)
0 (Enzyme Inhibitors)
0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
-
Entry Date(s):
-
Date Created: 20210620 Date Completed: 20210719 Latest Revision: 20210719
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.chroma.2021.462305
-
PMID:
-
34147833
-
Indoleamine 2,3-dioxygenase 1 (IDO1) has been shown to play an important role in the immune escape process of tumors, and therefore is considered as a promising target for tumor immunotherapy. In this study, off-line and on-line capillary electrophoresis methods were developed for IDO1 inhibitors screening from natural product extracts. The optimized separation conditions of CE were achieved with 32 mM sodium tetraborate (pH 9.22) as background electrolyte, using a separation voltage of 21 kV. The off-line CE method was verified by the determination of enzymatic kinetic parameters and inhibitory mechanisms of two known inhibitors. A partial filling on-line CE method combined with rapid polarity switching was used for rapid screening of IDO1 inhibitors. The whole reaction and separation process was completed within 5 min. The on-line CE screening results showed that six of 18 natural products had inhibitory effect on IDO1, namely Carthamus tinctorius, Schisandra chinensis, Raisin, Coffee, Hawthorn and Radix angelicae sinensis. The results of on-line CE experiments were consistent with the off-line results, which proved the practicability and effectiveness of the method for inhibitors screening.
Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
