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Tytuł:
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Changes in the Gut Microbiota Following Bariatric Surgery Are Associated with Increased Alcohol Intake in a Female Rat Model.
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Autorzy:
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Martin OA; Institute for Genome Sciences, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.; Department of Surgery, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.
Grant-Beurmann S; Institute for Genome Sciences, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.
Orellana ER; Department of Neural and Behavioral Sciences, Pennsylvania State University College of Medicine, University Drive. 10733, Hershey, PA, USA.
Hajnal A; Department of Neural and Behavioral Sciences, Pennsylvania State University College of Medicine, University Drive. 10733, Hershey, PA, USA.
Fraser CM; Institute for Genome Sciences, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.; Department of Medicine, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.; Department of Microbiology and Immunology, University of Maryland School of Medicine, West Baltimore St. 21201, Baltimore, MD, USA.
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Źródło:
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Alcohol and alcoholism (Oxford, Oxfordshire) [Alcohol Alcohol] 2021 Aug 30; Vol. 56 (5), pp. 605-613.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: <2003- > : Oxford : Oxford University Press
Original Publication: Oxford ; New York : Pergamon Press, c1983-
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MeSH Terms:
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Bariatric Surgery*
Ethanol/*administration & dosage
Gastrointestinal Microbiome/*physiology
Animals ; Female ; Gastrointestinal Microbiome/genetics ; Models, Animal ; Molecular Sequence Data ; Random Allocation ; Rats
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Grant Information:
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SAP # 4100077246 Pennsylvania Department of Health; University of Maryland; University of Maryland Foundation
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Substance Nomenclature:
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3K9958V90M (Ethanol)
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Entry Date(s):
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Date Created: 20210622 Date Completed: 20211206 Latest Revision: 20211214
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Update Code:
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20240105
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DOI:
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10.1093/alcalc/agab041
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PMID:
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34155502
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Aims: We aimed to investigate if differences in gut microbiota diversity and composition are associated with post-operative alcohol intake following bariatric surgery in a rat model.
Methods: Twenty-four female rats were randomized to three treatment groups: sham surgery, vertical sleeve gastrectomy (VSG) or Roux-en-Y gastric bypass (RYGB). Stool was collected pre- and post-operatively and 16S rRNA gene amplification and sequencing was performed. Analysis focused on correlating microbial diversity, type of surgery and alcohol (EtOH) intake.
Results: Pre-operative stools samples on regular diet showed similar taxonomic composition and Shannon diversity among the three treatment groups. There was a significant decrease in Shannon diversity and a change in taxonomic composition of the gut microbiota after rats was fed high fat diet. Post-operatively, the RYGB group showed significantly lower taxonomic diversity than the VSG and sham groups, while the VSG and sham groups diversity were not significantly different. Taxonomic composition and function prediction based on PICRUSt analysis showed the RYGB group to be distinct from the VSG and sham groups. Shannon diversity was found to be negatively associated with EtOH intake.
Conclusions: Changes in the taxonomic profile of the gut microbiota following bariatric surgery, particularly RYGB, are associated with increased EtOH intake and may contribute to increased alcohol use disorder risk through the gut-brain-microbiome axis.
(© The Author(s) 2021. Medical Council on Alcohol and Oxford University Press. All rights reserved.)
