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Tytuł:
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The utility of a single tube 10-color flow cytometry for quantitative and qualitative analysis in myelodysplastic syndrome- a pilot study.
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Autorzy:
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Chauhan R; Department of Hematology, Sir Ganga Ram Hospital, New Delhi, India.
Singh J; Department of Laboratory Oncology, B.R.A.I.R.C.H., AIIMS, New Delhi, India.
Sharma C; Department of Mathematics, Shiv Nadar University, Noida, U.P, India.
Dange P; Department of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Chopra A; Department of Laboratory Oncology, B.R.A.I.R.C.H., AIIMS, New Delhi, India. Electronic address: .
Mahapatra M; Department of Hematology, All India Institute of Medical Sciences, New Delhi, India.
Pati H; Department of Hematology, All India Institute of Medical Sciences, New Delhi, India.
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Źródło:
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Leukemia research [Leuk Res] 2021 Aug; Vol. 107, pp. 106651. Date of Electronic Publication: 2021 Jun 29.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Oxford ; New York : Pergamon Press
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MeSH Terms:
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Biomarkers*
Immunophenotyping*/methods
Myelodysplastic Syndromes/*diagnosis
Myelodysplastic Syndromes/*metabolism
Antigens, CD/metabolism ; Flow Cytometry ; Humans ; Leukocytes/metabolism ; Myelodysplastic Syndromes/etiology ; Pilot Projects ; Prognosis
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Contributed Indexing:
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Keywords: 10-color; Flow cytometry; Myelodysplastic syndrome; Single-tube
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Substance Nomenclature:
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0 (Antigens, CD)
0 (Biomarkers)
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Entry Date(s):
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Date Created: 20210704 Date Completed: 20210830 Latest Revision: 20210830
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Update Code:
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20240105
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DOI:
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10.1016/j.leukres.2021.106651
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PMID:
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34218155
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Introduction: Assessment of myelodysplasia (MDS) by flow cytometry (FCM) includes elaborate panels, and interpretation is observer-dependent. This study evaluates single tube 10-color FCM in a test cohort of clinically suspected MDS patients.
Methods: We analyzed fifty-six bone marrow (BM) samples from clinically suspected MDS patients in a morphology-blinded manner along with controls using a 10-color single tube flow cytometry. We analyzed the reproducibility of Ogata score and modified FCM scores, additionally incorporating the proportion of CD15, CD11b, CD56, and CD38MFI on CD34+CD19-cluster for each patient. Patients were grouped as proven-MDS, suspected-MDS, and non-MDS groups based on morphology and cytogenetics. Optimized multi-axial radar-plots were also used to analyze maturation patterns in the granulocytic, monocytic, and blast progenitor compartments of proven-MDS cases and controls.
Results: Flow cytometric abnormalities ≥3 were present in proven-MDS (n = 23) with a sensitivity and specificity of 78 % and 94 %, respectively, as per Ogata score. The addition of CD38 MFI to the score yielded sensitivity and specificity of 82 % and 88 %, respectively. Additional analysis of aberrant expression of CD15, CD11b, and CD56 increased the diagnostic power of the FCM score. A qualitative analysis of data also showed differences in maturation patterns in proven-MDS compared to the control group.
Conclusion: Single tube 10-color FCM scoring, including Ogata score, modified-FCM scores, and radar plots pattern analysis, showed significant abnormalities in proven-MDS cases in this pilot study. Large databases, including FCM-scoring and pattern-based analysis for normal BM maturation, could be further validated and standardized for screening MDS.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
