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Tytuł:
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3D printed PCL/β-TCP cross-scale scaffold with high-precision fiber for providing cell growth and forming bones in the pores.
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Autorzy:
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Wang Q; School of Mechanical Engineering & Mechanics, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Ye W; School of Mechatronics & Vehicle Engineering, East China Jiaotong University, Nanchang 330013, PR China.
Ma Z; School of Engineering, Huzhou University, Huzhou, Zhejiang 313000, PR China. Electronic address: .
Xie W; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610000, PR China.
Zhong L; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610000, PR China.
Wang Y; School of Mechanical Engineering & Mechanics, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Rong Q; Department of Stomatology, the First People's Hospital of Yunnan Province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, PR China. Electronic address: .
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Źródło:
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Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2021 Aug; Vol. 127, pp. 112197. Date of Electronic Publication: 2021 May 24.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier
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MeSH Terms:
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Osteogenesis*
Polyesters*
Bone and Bones ; Calcium Phosphates ; Printing, Three-Dimensional ; Tissue Engineering ; Tissue Scaffolds
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Contributed Indexing:
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Keywords: 3D printing; Bone tissue engineering; Cross-scale scaffold; PCL; β-TCP
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Substance Nomenclature:
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0 (Calcium Phosphates)
0 (Polyesters)
0 (beta-tricalcium phosphate)
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Entry Date(s):
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Date Created: 20210706 Date Completed: 20210707 Latest Revision: 20210707
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Update Code:
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20240105
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DOI:
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10.1016/j.msec.2021.112197
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PMID:
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34225850
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Scaffolds prepared by 3D printing are increasingly used in the field of bone tissue repair. However, on traditional 3D printed bone tissue engineering scaffolds, cells can only grow on the fiber surface and form bone. We designed a scaffold with a cross-scale structure of PCL/β-TCP, which contains thick fibers with a diameter of 500 μm printed by FDM. And in the pores of the coarse fiber, the ultra-high precision fine fiber grid with a diameter of about 10 μm is filled by MEW mode. In cell experiments, cells can not only grow on the thick fiber surface of the cross-scale scaffold. At the same time, the mesh structure of fine fibers provides a bridge for cell growth, allowing cells to pass through the pores of thick fibers and grow in the pores and gradually cover the pores of the scaffold. In the osteoinduction experiment, β-TCP in the PCL/β-TCP composite provides Ca 2+ and PO 4 3- to the scaffold, which effectively promotes the osteogenic differentiation of cells on the scaffold. Compared with traditional scaffolds, the osteogenic performance of cross-scale scaffolds is greatly improved. Not only did bone form on the surface of the scaffold, but also obvious ALP expression and effective calcium precipitation appeared in the pores of the scaffold. This can effectively speed up the repair of bone defects. We believe that the 3D printed PCL/β-TCP cross-scale scaffold with high-precision fibers has great application prospects in the field of bone tissue engineering.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
