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Tytuł:
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The global profiling of alkaloids in Aconitum stapfianum and analysis of detoxification material basis against Fuzi.
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Autorzy:
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Liu S; School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China.
Lai C; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijng 100700, China.
Long Y; School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China.
Yang W; School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China. Electronic address: .
Ren Q; Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center of Drug and Medical Device Quality, Nanchang 330029, China.
Huang L; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijng 100700, China. Electronic address: .
Chen J; School of Resources Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China; Center for Post-doctoral research, China Academy of Chinese Medical Sciences, Beijng 100700, China. Electronic address: .
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Źródło:
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Journal of chromatography. A [J Chromatogr A] 2021 Aug 30; Vol. 1652, pp. 462362. Date of Electronic Publication: 2021 Jun 28.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam ; New York : Elsevier, 1993-
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MeSH Terms:
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Aconitum*/chemistry
Alkaloids*/analysis
Alkaloids*/chemistry
Diterpenes*/chemistry
Drug Interactions*
Drugs, Chinese Herbal*/chemistry
Chromatography, High Pressure Liquid ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Roots/chemistry
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Contributed Indexing:
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Keywords: Aconitum stapfianum; Alkaloids; Detoxification; Fragmentation mechanism; Multicomponent characterization
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Substance Nomenclature:
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0 (Alkaloids)
0 (Diterpenes)
0 (Drugs, Chinese Herbal)
0 (Plant Extracts)
0 (fuzi drug herbal)
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Entry Date(s):
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Date Created: 20210714 Date Completed: 20211006 Latest Revision: 20211006
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Update Code:
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20240105
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DOI:
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10.1016/j.chroma.2021.462362
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PMID:
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34261022
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Aconitum alkaloids are versatile in chemical structures and are well known for their bioactivity and toxicity. Cases of analogs with closely similar structures or positional isomers are widespread in herbs of the Aconitum genus. It is still challenging to rapidly identify unknown compounds via mass spectrometry, especially positional isomers. Herein, to profile the alkaloids of Aconitum stapfianum that possess bioactivity against intoxication by the lateral root of Aconitum carmichaelii (Fuzi), a strategy was developed by carefully determining the fragmentation pathways of authentic standards. A series of rules was summarized and involved charge site effects, hydrogen bonding effects, competitive channels between charge-remote reactions and charge migration reactions, and fragment patterns exhibiting a "diamond shape". Accordingly, a total of 124 alkaloids in A. stapfianum were tentatively characterized, including 85 potential new compounds and 24 sets of isomers. On the other hand, to explore the material basis of detoxification, a chemical constituent comparison was made between A. stapfianum and Fuzi, and principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to identify markers that were different between the two . In total, 25 characteristic markers were identified to discriminate between these two herbal medicines, of which 14 compounds were specific for A. stapfianum and most of them were characteristic for a para-substituted benzoic acid ester at C-14.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
