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Tytuł:
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Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens.
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Autorzy:
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Bonura F; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
Mascarella C; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
Filizzolo C; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
Bonura C; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
Ferraro D; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
Di Bernardo F; Unità Operativa di Microbiologia e Virologia, Ospedale Civico e Di Cristina, ARNAS, Palermo, Italy.
Collura A; Unità Operativa di Microbiologia e Virologia, Ospedale Civico e Di Cristina, ARNAS, Palermo, Italy.
Martella V; Dipartimento di Medicina Veterinaria, Università Aldo Moro di Bari, Valenzano, Italy.
Giammanco GM; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy.
De Grazia S; Dipartimento di Scienze per la Promozione della Salute e Materno Infantile 'G. D'Alessandro', Palermo, Italy. Electronic address: .
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Źródło:
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Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2021 Nov; Vol. 101 (3), pp. 115459. Date of Electronic Publication: 2021 Jun 20.
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Typ publikacji:
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Evaluation Study; Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: [New York, NY] : Elsevier Biomedical, [c1983-
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MeSH Terms:
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Adenoviridae/*immunology
Adenoviridae Infections/*diagnosis
Antigens, Viral/*immunology
Gastroenteritis/*diagnosis
Gastroenteritis/*virology
Luminescent Measurements/*standards
Reagent Kits, Diagnostic/*standards
Adenoviridae/genetics ; Adolescent ; Antigens, Viral/genetics ; Child ; Child, Preschool ; Feces/virology ; Hospitalization ; Humans ; Infant ; Infant, Newborn ; Italy ; Luminescent Measurements/methods ; Sensitivity and Specificity
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Contributed Indexing:
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Keywords: Acute gastroenteritis; Chemiluminescent immunoassay; Diagnostic performances; Enteric adenoviruses; Fecal antigens detection; Immuno chromatography
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Substance Nomenclature:
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0 (Antigens, Viral)
0 (Reagent Kits, Diagnostic)
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Entry Date(s):
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Date Created: 20210719 Date Completed: 20220113 Latest Revision: 20220113
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Update Code:
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20240105
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DOI:
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10.1016/j.diagmicrobio.2021.115459
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PMID:
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34280672
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The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90.9) and concordance (k: 0.81 vs 0.67) with respect to ICT, despite equivalent specificity (98.8%). Using the Ct values of the Rt-PCR as a proxy of the fecal viral load, similar Ct values (mean 9.32 vs 9.89) were observed among the true positive samples, whilst a significant difference (P < 0.05) was observed in false negative samples of CLIA (mean Ct 25.68) and ICT (mean Ct 19.87). Cross-reactivity with other enteric viruses was not observed. These results indicate that both the assays tested are suitable for diagnosis of AdV gastroenteritis.
Competing Interests: Conflict of interest The authors declare that they have no conflict of interest.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
