-
Tytuł:
-
Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction.
-
Autorzy:
-
Cesare MM; Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.; Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.
Felice F; Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.
Conti V; Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.
Cerri L; Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.; Department of Pharmacy, University of Pisa, 56126 Pisa, Italy.
Zambito Y; Department of Pharmacy, University of Pisa, 56126 Pisa, Italy.; Interdepartmental Research Center, Nutraceuticals and Food for Health, University of Pisa, 56100 Pisa, Italy.
Romi M; Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.
Cai G; Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy.
Cantini C; CNR-IBE (Consiglio Nazionale delle Ricerche-Istituto per la Bioeconomia), 58022 Follonica, Italy.
Di Stefano R; Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, Italy.; Interdepartmental Research Center, Nutraceuticals and Food for Health, University of Pisa, 56100 Pisa, Italy.; S.D. of Sport Medicine, Pisa University Hospital, 56100 Pisa, Italy.
-
Źródło:
-
Molecules (Basel, Switzerland) [Molecules] 2021 Jul 15; Vol. 26 (14). Date of Electronic Publication: 2021 Jul 15.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Basel, Switzerland : MDPI, c1995-
-
MeSH Terms:
-
Antioxidants/*pharmacology
Endothelial Cells/*drug effects
Solanum lycopersicum/*chemistry
Plant Extracts/*pharmacology
Polyphenols/*pharmacology
Antioxidants/chemistry ; Droughts ; Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells ; Humans ; Solanum lycopersicum/physiology ; Oxidative Stress/drug effects ; Plant Extracts/chemistry ; Polyphenols/chemistry ; Stress, Physiological
-
References:
-
J Sci Food Agric. 2018 Mar;98(4):1271-1279. (PMID: 28799642)
J Appl Toxicol. 2017 Dec;37(12):1359-1369. (PMID: 28383141)
Molecules. 2010 Feb 23;15(2):959-87. (PMID: 20335956)
J Sep Sci. 2008 Feb;31(2):262-7. (PMID: 18172921)
Free Radic Biol Med. 2004 Apr 15;36(8):1058-67. (PMID: 15059646)
Plant Physiol Biochem. 2016 Aug;105:79-89. (PMID: 27085599)
Mol Nutr Food Res. 2007 May;51(5):609-17. (PMID: 17427261)
Br J Pharmacol. 2017 Jun;174(11):1290-1324. (PMID: 27638711)
Int J Pharm. 2005 Mar 3;291(1-2):183-8. (PMID: 15707745)
Crit Rev Food Sci Nutr. 2015;55(7):919-29. (PMID: 24915375)
Int J Prev Med. 2014 Dec;5(12):1487-99. (PMID: 25709784)
J Agric Food Chem. 2007 Aug 8;55(16):6475-81. (PMID: 17629300)
Plants (Basel). 2019 Sep 07;8(9):. (PMID: 31500309)
J Agric Food Chem. 2007 Oct 17;55(21):8604-15. (PMID: 17927150)
Anal Biochem. 1996 Jul 15;239(1):70-6. (PMID: 8660627)
J Med Food. 2013 Apr;16(4):351-6. (PMID: 23472745)
Curr Opin Biotechnol. 2010 Apr;21(2):197-203. (PMID: 20363612)
Plant Physiol Biochem. 2009 Jul;47(7):570-7. (PMID: 19318268)
J Sci Food Agric. 2014 Mar 30;94(5):993-9. (PMID: 24037905)
Am J Clin Nutr. 2012 May;95(5):1013-22. (PMID: 22492370)
Nutrients. 2015 Nov 19;7(11):9640-9. (PMID: 26610560)
Front Plant Sci. 2014 Mar 13;5:86. (PMID: 24659993)
Eur J Pharm Sci. 2018 Jan 1;111:425-431. (PMID: 29055735)
Nutrients. 2020 Aug 28;12(9):. (PMID: 32872374)
Front Pharmacol. 2018 May 23;9:521. (PMID: 29875663)
Crit Rev Food Sci Nutr. 2014;54(8):1032-49. (PMID: 24499120)
Front Plant Sci. 2016 Jul 14;7:1029. (PMID: 27471513)
Foods. 2017 Jul 25;6(8):. (PMID: 28757563)
Oxid Med Cell Longev. 2013;2013:516051. (PMID: 23577223)
J Agric Food Chem. 2013 Oct 9;61(40):9534-50. (PMID: 24079774)
Eur J Pharm Biopharm. 2010 Jun;75(2):194-9. (PMID: 20171277)
Antioxidants (Basel). 2020 Feb 03;9(2):. (PMID: 32028655)
Int J Food Sci Nutr. 2009 Dec;60(8):694-708. (PMID: 19919517)
J Sep Sci. 2007 Mar;30(4):452-61. (PMID: 17444214)
J Clin Invest. 1973 Nov;52(11):2745-56. (PMID: 4355998)
Oxid Med Cell Longev. 2019 Jun 23;2019:7092151. (PMID: 31341533)
Nutrients. 2018 Nov 01;10(11):. (PMID: 30388730)
J Zhejiang Univ Sci B. 2010 May;11(5):357-65. (PMID: 20443214)
Int J Mol Sci. 2019 Nov 25;20(23):. (PMID: 31775339)
Ann Nutr Metab. 2003;47(2):64-9. (PMID: 12652057)
Rapid Commun Mass Spectrom. 2010 Oct 30;24(20):2986-92. (PMID: 20872631)
Eur J Pharm Biopharm. 2012 Jan;80(1):176-84. (PMID: 21971368)
Plant Foods Hum Nutr. 2018 Dec;73(4):268-277. (PMID: 30264237)
J Agric Food Chem. 2011 Sep 14;59(17):9673-82. (PMID: 21830786)
J Sci Food Agric. 2013 Apr;93(6):1449-57. (PMID: 23070982)
-
Contributed Indexing:
-
Keywords: drought stress; human vascular endothelial cells; oxidative stress; polyphenols; tomato by-products; tomato peel extract
-
Substance Nomenclature:
-
0 (Antioxidants)
0 (Plant Extracts)
0 (Polyphenols)
-
Entry Date(s):
-
Date Created: 20210724 Date Completed: 20210927 Latest Revision: 20221207
-
Update Code:
-
20240105
-
PubMed Central ID:
-
PMC8307906
-
DOI:
-
10.3390/molecules26144289
-
PMID:
-
34299564
-
Background: Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction.
Methods: The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H 2 O 2 . Cell viability, ROS production and CAT, SOD and GPx activities were evaluated. Permeation of antioxidant molecules contained in RED across excised rat intestine was also studied.
Results: RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H 2 O 2 -induced oxidative stress and significantly reduced ROS production raising SOD and CAT activity (* p < 0.05 and ** p < 0.005 vs. H 2 O 2 , respectively). The permeation of antioxidant molecules contained in RED-Ctr or RED-Ds across excised rat intestine was high with non-significant difference between the two RED types (41.9 ± 9.6% vs. 26.6 ± 7.8%).
Conclusions: RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.