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Tytuł pozycji:

Epigenetic modifications underlie the differential adipogenic potential of preadipocytes derived from human subcutaneous fat tissue.

Tytuł:
Epigenetic modifications underlie the differential adipogenic potential of preadipocytes derived from human subcutaneous fat tissue.
Autorzy:
Kubota Y; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Nagano H; Department of Molecular Diagnosis, Chiba University, Chiba-city, Chiba, Japan.
Kosaka K; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Ogata H; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Nakayama A; Department of Molecular Diagnosis, Chiba University, Chiba-city, Chiba, Japan.
Yokoyama M; Department of Molecular Diagnosis, Chiba University, Chiba-city, Chiba, Japan.
Murata K; Department of Molecular Diagnosis, Chiba University, Chiba-city, Chiba, Japan.
Akita S; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Kuriyama M; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Furuyama N; Jiyugaoka Clinic, Tokyo, Japan.
Kuroda M; Center for Advanced Medicine, Chiba University, Chiba-city, Chiba, Japan.
Tanaka T; Department of Molecular Diagnosis, Chiba University, Chiba-city, Chiba, Japan.
Mitsukawa N; Department of Plastic Surgery, Chiba University, Chiba-city, Chiba, Japan.
Źródło:
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2021 Sep 01; Vol. 321 (3), pp. C596-C606. Date of Electronic Publication: 2021 Jul 28.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: Bethesda, Md. : American Physiological Society,
MeSH Terms:
Epigenesis, Genetic*
Adipocytes/*metabolism
Adipogenesis/*genetics
Adipokines/*genetics
Mesenchymal Stem Cells/*metabolism
PPAR gamma/*genetics
Adipocytes/classification ; Adipocytes/cytology ; Adipokines/metabolism ; CpG Islands ; DNA Methylation ; Fatty Acid-Binding Proteins/genetics ; Fatty Acid-Binding Proteins/metabolism ; Female ; Gene Expression Profiling ; Genome-Wide Association Study ; Histones/genetics ; Histones/metabolism ; Humans ; Leptin/genetics ; Leptin/metabolism ; Mammaplasty/methods ; Mammary Glands, Human/cytology ; Mammary Glands, Human/metabolism ; Mammary Glands, Human/surgery ; Mesenchymal Stem Cells/classification ; Mesenchymal Stem Cells/cytology ; Organ Specificity ; PPAR gamma/metabolism ; Primary Cell Culture ; Subcutaneous Fat/cytology ; Subcutaneous Fat/metabolism ; Unsupervised Machine Learning
Contributed Indexing:
Keywords: adipocyte; differentiation; epigenetics; histone; methylation
Substance Nomenclature:
0 (Adipokines)
0 (FABP4 protein, human)
0 (Fatty Acid-Binding Proteins)
0 (Histones)
0 (LEP protein, human)
0 (Leptin)
0 (PPAR gamma)
0 (PPARG protein, human)
Entry Date(s):
Date Created: 20210728 Date Completed: 20210921 Latest Revision: 20210921
Update Code:
20240105
DOI:
10.1152/ajpcell.00387.2020
PMID:
34319829
Czasopismo naukowe
Ceiling culture-derived preadipocytes (ccdPAs) and adipose-derived stem cells (ASCs) can be harvested from human subcutaneous fat tissue using the specific gravity method. Both cell types possess a similar spindle shape without lipid droplets. We previously reported that ccdPAs have a higher adipogenic potential than ASCs, even after a 7-wk culture. We performed a genome-wide epigenetic analysis to examine the mechanisms contributing to the adipogenic potential differences between ccdPAs and ASCs. Methylation analysis of cytosines followed by guanine (CpG) using a 450-K BeadChip was performed on human ccdPAs and ASCs isolated from three metabolically healthy females. Chromatin immunoprecipitation sequencing was performed to evaluate trimethylation at lysine 4 of histone 3 (H3K4me3). Unsupervised machine learning using t-distributed stochastic neighbor embedding to interpret 450,000-dimensional methylation assay data showed that the cells were divided into ASC and ccdPA groups. In Kyoto Encyclopedia of Genes and Genomes pathway analysis of 1,543 genes with differential promoter CpG methylation, the peroxisome proliferator-activated receptor (PPAR) and adipocytokine signaling pathways ranked in the top 10 pathways. In the PPARγ gene, H3K4me3 peak levels were higher in ccdPAs than in ASCs, whereas promoter CpG methylation levels were significantly lower in ccdPAs than in ASCs. Similar differences in promoter CpG methylation were also seen in the fatty acid-binding protein 4 and leptin genes. In conclusion, we analyzed the epigenetic status of adipogenesis-related genes as a potential mechanism underlying the differences in adipogenic differentiation capability between ASCs and ccdPAs.

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