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Tytuł:
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Acoustic Ejection Mass Spectrometry for High-Throughput Analysis.
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Autorzy:
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Zhang H; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Liu C; SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4V8, Canada.
Hua W; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Ghislain LP; Beckman Coulter Life Sciences Inc., San Jose, California 95134, United States.
Liu J; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Aschenbrenner L; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Noell S; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Dirico KJ; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Lanyon LF; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Steppan CM; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
West M; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
Arnold DW; SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4V8, Canada.
Covey TR; SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4V8, Canada.
Datwani SS; Beckman Coulter Life Sciences Inc., San Jose, California 95134, United States.
Troutman MD; Pfizer Global Research and Development, Eastern Point Road, Groton, Connecticut 06340, United States.
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Źródło:
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Analytical chemistry [Anal Chem] 2021 Aug 10; Vol. 93 (31), pp. 10850-10861. Date of Electronic Publication: 2021 Jul 28.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, American Chemical Society.
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MeSH Terms:
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High-Throughput Screening Assays*
Spectrometry, Mass, Electrospray Ionization*
Acoustics
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Entry Date(s):
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Date Created: 20210728 Date Completed: 20210831 Latest Revision: 20210831
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Update Code:
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20240105
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DOI:
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10.1021/acs.analchem.1c01137
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PMID:
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34320311
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We describe a mass spectrometry (MS) analytical platform resulting from the novel integration of acoustic droplet ejection (ADE) technology, an open-port interface (OPI), and electrospray ionization (ESI)-MS that creates a transformative system enabling high-speed sampling and label-free analysis. The ADE technology delivers nanoliter droplets in a touchless manner with high speed, precision, and accuracy. Subsequent sample dilution within the OPI, in concert with the capabilities of modern ESI-MS, eliminates the laborious sample preparation and method development required in current approaches. This platform is applied to a variety of experiments, including high-throughput (HT) pharmacology screening, label-free in situ enzyme kinetics, in vitro absorption, distribution, metabolism, elimination, pharmacokinetic and biomarker analysis, and HT parallel medicinal chemistry.
