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Tytuł:
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Real-time monitoring of active caspase 3 during AFB1 induced apoptosis based on SERS-fluorescent dual mode signals.
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Autorzy:
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Ma X; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, PR China; Collaborative Innovation Center of Food Safety And Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, PR China.
Zhang J; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
Wang Z; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, PR China; Collaborative Innovation Center of Food Safety And Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, PR China. Electronic address: .
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Źródło:
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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2021 Dec 15; Vol. 263, pp. 120195. Date of Electronic Publication: 2021 Jul 17.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: : Amsterdam : Elsevier
Original Publication: [Kidlington, Oxford, U.K. ; Tarrytown, NY] : Pergamon, c1994-
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MeSH Terms:
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Aflatoxin B1*/analysis
Aflatoxin B1*/toxicity
Metal Nanoparticles*/toxicity
Apoptosis ; Caspase 3 ; Gold ; Humans ; Spectrum Analysis, Raman
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Contributed Indexing:
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Keywords: AFB1; AuNTs; Caspase 3; Fluorescence; SERS
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Substance Nomenclature:
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7440-57-5 (Gold)
9N2N2Y55MH (Aflatoxin B1)
EC 3.4.22.- (Caspase 3)
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Entry Date(s):
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Date Created: 20210730 Date Completed: 20210906 Latest Revision: 20210906
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Update Code:
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20240105
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DOI:
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10.1016/j.saa.2021.120195
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PMID:
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34329847
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Aflatoxin B1 (AFB1) is the most toxic mycotoxin. Usually, the toxin activated apoptosis is considered mostly through intrinsic mitochondrial pathway while the caspase family as promoter and executor plays a crucial role. In this paper, a real-time and in situ detection of caspase 3 in living cells based on SERS-fluorescence dual mode nanosensor was studied. Firstly, gold nanotriangles (AuNTs) modified with the caspase 3 specifically recognized polypeptide chain DEVD were synthesized as both SERS enhanced substrate and fluorescent quencher. Rhodamine B (Rb) as both Raman and fluorescent signal molecules was modified on the N end of DEVD chain. After active caspase 3 specifically cut off the recognition site in DEVD, partial peptide chain with Rb fell off from the surface of AuNTs. Thus, the Raman signal of Rb decreased while its fluorescent signal recovered. There was a good linear relationship between caspase 3 and both the SERS and fluorescence signals of Rb. The minimum detection limit was 0.001 nM. After cells were stimulated by AFB1, when Cyt C in the cytoplasm reached a certain level, caspase 3 was activated. This nanosensor was realized in certain living cells (HepG2, HeLa and A549). Based on monitoring the activation of specific apoptotic markers, the conduction of marker signals in real time can provide more detailed information for apoptosis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
