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Tytuł:
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Generation of a 100-billion cyclic peptide phage display library having a high skeletal diversity.
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Autorzy:
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Carle V; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
Kong XD; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
Comberlato A; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
Edwards C; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
Díaz-Perlas C; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
Heinis C; Institute of Chemical Sciences and Engineering, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland.
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Źródło:
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Protein engineering, design & selection : PEDS [Protein Eng Des Sel] 2021 Feb 15; Vol. 34.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: Oxford, UK : Oxford University Press, c2003-
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MeSH Terms:
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Peptide Library*
Peptides*/genetics
Ligands ; Peptides, Cyclic ; Plasmids
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Contributed Indexing:
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Keywords: bicyclic peptide; cyclic peptide; library; phage display; whole plasmid PCR
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Substance Nomenclature:
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0 (Ligands)
0 (Peptide Library)
0 (Peptides)
0 (Peptides, Cyclic)
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Entry Date(s):
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Date Created: 20210803 Date Completed: 20211028 Latest Revision: 20211028
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Update Code:
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20240105
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DOI:
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10.1093/protein/gzab018
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PMID:
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34341825
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Phage display is a powerful technique routinely used for the generation of peptide- or protein-based ligands. The success of phage display selections critically depends on the size and structural diversity of the libraries, but the generation of large libraries remains challenging. In this work, we have succeeded in developing a phage display library comprising around 100 billion different (bi)cyclic peptides and thus more structures than any previously reported cyclic peptide phage display library. Building such a high diversity was achieved by combining a recently reported library cloning technique, based on whole plasmid PCR, with a small plasmid that facilitated bacterial transformation. The library cloned is based on 273 different peptide backbones and thus has a large skeletal diversity. Panning of the peptide repertoire against the important thrombosis target coagulation factor XI enriched high-affinity peptides with long consensus sequences that can only be found if the library diversity is large.
(© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
