Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties.

Tytuł:: Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties.
Autorzy:: Kanazawa S; Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Okada H; Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hojo H; Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.; Department of Bioengineering, Facility of Medicine, The University of Tokyo, Tokyo, Japan.
Ohba S; Department of Cell Biology, Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Iwata J; Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center At Houston, Houston, TX, USA.
Komura M; Division of Tissue Engineering, The University of Tokyo Hospital, Tokyo, Japan.
Hikita A; Department of Cell and Tissue Engineering (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Hoshi K; Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. .
Źródło:: Scientific reports [Sci Rep] 2021 Aug 04; Vol. 11 (1), pp. 15811. Date of Electronic Publication: 2021 Aug 04.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Epigenesis, Genetic*
Gene Expression Regulation*
Stem Cell Niche*
Transcriptome*
Bone Marrow Cells/*metabolism
Mesenchymal Stem Cells/*metabolism
Single-Cell Analysis/*methods
Animals ; Bone Marrow Cells/cytology ; Cell Differentiation ; Cell Lineage ; Gene Expression Profiling ; Gene Regulatory Networks ; Male ; Mesenchymal Stem Cells/cytology ; Mice ; Mice, Inbred C57BL ; Signal Transduction
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Entry Date(s):: Date Created: 20210805 Date Completed: 20211108 Latest Revision: 20211108
Update Code:: 20240105
PubMed Central ID:: PMC8338933
DOI:: 10.1038/s41598-021-94186-5
PMID:: 34349154
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Pełny tekst

Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies and properties causes difficulties in molecular separation of their distinct populations. In this study, by taking advantage of the resolution of the single cell transcriptome, we analyzed Sca-1 and PDGFR-α fraction in the mouse bone marrow tissue. The single cell transcriptome enabled us to further classify the population into seven populations according to their gene expression profiles. We then separately obtained the seven populations based on candidate marker genes, and specified their gene expression properties and epigenetic landscape by ATAC-seq. Our findings will enable to elucidate the stem cell niche signal in the bone marrow microenvironment, reconstitute bone marrow in vitro, and shed light on the potentially important role of identified subpopulation in various clinical applications to the treatment of bone- and bone marrow-related diseases.
(© 2021. The Author(s).)

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