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Tytuł pozycji:

Proteases and variants: context matters for SARS-CoV-2 entry assays.

Tytuł:
Proteases and variants: context matters for SARS-CoV-2 entry assays.
Autorzy:
Stevens CS; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, United States.
Oguntuyo KY; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, United States.
Lee B; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, United States. Electronic address: .
Źródło:
Current opinion in virology [Curr Opin Virol] 2021 Oct; Vol. 50, pp. 49-58. Date of Electronic Publication: 2021 Jul 24.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Review
Język:
English
Imprint Name(s):
Original Publication: Amsterdam : Elsevier
MeSH Terms:
Virus Internalization*
COVID-19/*etiology
Peptide Hydrolases/*physiology
SARS-CoV-2/*physiology
Humans ; Mutation ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/genetics
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Grant Information:
T32 AI007647 United States AI NIAID NIH HHS; R01 AI123449 United States AI NIAID NIH HHS; R01 AI138921 United States AI NIAID NIH HHS; R01 AI071002 United States AI NIAID NIH HHS; R01 AI125536 United States AI NIAID NIH HHS; R21 AI115226 United States AI NIAID NIH HHS; F31 AI154739 United States AI NIAID NIH HHS
Substance Nomenclature:
0 (Spike Glycoprotein, Coronavirus)
0 (spike protein, SARS-CoV-2)
EC 3.4.- (Peptide Hydrolases)
Entry Date(s):
Date Created: 20210808 Date Completed: 20211018 Latest Revision: 20231107
Update Code:
20240105
PubMed Central ID:
PMC8302850
DOI:
10.1016/j.coviro.2021.07.004
PMID:
34365113
Czasopismo naukowe
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), like other coronaviruses, relies on a flexible array of entry mechanisms, driven by the spike (S) protein. Entry is dependent on proteolytic priming, activation, and receptor binding; all of which can be variable, dependent on context. Here we review the implications of the complexity of SARS-CoV-2 entry pathways on entry assays that then drive our understanding of humoral immunity, therapeutic efficacy, and tissue restriction. We focus especially on the proteolytic activation of SARS-CoV-2 spike and how this constellation of proteases lends deeper insight to our understanding of arising variants and their putative role transmission or variable pathogenicity in vivo. In this review, we argue for better universal standards to assay virus entry as well as suggest best practices for reporting viral passage number, the cell line used, and proteases present, among other important considerations.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

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